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High-resolution imaging of cellular proteins : methods and protocols / edited by Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski.
Format
Book
Language
English
Published/Created
New York, NY : Humana Press, 2016.
Description
1 online resource (xiii, 366 pages) : illustrations (some color).
Availability
Available Online
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1474
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Details
Subject(s)
Electron microscopy
—
Laboratory manuals
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Editor
Schwartzbach, Steven D.
[Browse]
Skalli, Omar
[Browse]
Schikorski, Thomas
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1474.
[More in this series]
Methods in molecular biology, 1064-3745 ; 1474
Summary note
This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.
Notes
Includes index.
Bibliographic references
Includes bibliographical references and index.
Source of description
Online resource; title from PDF title page (SpringerLink, viewed August 19, 2016).
Contents
Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture
Antibody Production with Synthetic Peptides
Production and Purification of Polyclonal Antibodies
Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2 and Streptavidin
Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications
Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy
Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells
Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses
Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells
Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging
Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins
Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas
Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells
Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues
Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae
Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes
Pre-Embedding Nanogold Silver and Gold Intensification
Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval
Pre- and Post-Embedding Immunogold Labeling of Tissue Sections
Immuno-Gold Labelling for Scanning Electron Microscopy
Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope
Functional Nanoscale Imaging Of Synaptic Vesicle Cycling With Superfast Fixation.
Show 19 more Contents items
ISBN
9781493963522 ((electronic bk.))
149396352X ((electronic bk.))
LCCN
2016946471
OCLC
956984895
Doi
10.1007/978-1-4939-6352-2
Statement on language in description
Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Other versions
High-Resolution Imaging of Cellular Proteins [electronic resource] : Methods and Protocols / edited by Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski.
id
99125306518206421