Details

Subject(s)

• Optical tweezers—Laboratory manuals [Browse]

Editor

• Gennerich, Arne [Browse]

Library of Congress genre(s)

• Laboratory manuals [Browse]

Series

• Methods in molecular biology (Clifton, N.J.) ; v. 1486. [More in this series]
• Springer protocols (Series) [More in this series]
• Methods in molecular biology, 1064-3745 ; 1486
• Springer protocols

Notes

"This Humana Press imprint is published by Springer Nature"--Title page verso.

Bibliographic references

Includes bibliographical references and index.

Contents

• Introduction to optical tweezers
• Exact theory of optical tweezers and its application to absolute calibration
• Beyond the hookean spring model: Direct measurement of optical forces through light momentum changes
• A surface-coupled optical trap with 1-bp precision via active stabilization
• Implementation and tuning of an optical tweezers force-clamp feedback system
• Custom-made microspheres for optical tweezers
• Optical torque wrench design and calibration
• High-resolution "fleezers": Dual-trap optical tweezers combined with single-molecule fluorescence detection
• Versatile quadruple-trap optical tweezers for dual DNA experiments
• Probing DNA-DNA interactions with a combination of quadruple-trap optical tweezers and microfluidics
• Probing single helicase dynamics on long nucleic acids through fluorescence-force measurement
• Mechanically watching the clpXP proteolytic machinery
• Deciphering the molecular mechanism of the bacteriophage phi29 DNA packaging motor
• Single-molecule protein folding experiments using high-precision optical tweezers
• Observing single RNA polymerase molecules down to base-pair resolution
• Optical tweezers-based measurements of forces and dynamics at microtubule ends
• Simultaneous manipulation and super-resolution fluorescence imaging of individual kinetochores coupled to microtubule tips
• Measurement of force-dependent release rates of cytoskeletal motors
• Measuring the kinetic and mechanical properties of non-processive myosins using optical tweezers
• Quantifying force and viscoelasticity inside living cells using an active-passive calibrated optical trap
• Measuring molecular forces using calibrated optical tweezers in living cells.

Show 18 more Contents items

Other format(s)

Also available in an electronic version.

ISBN

• 9781493964192 ((alk. paper))
• 1493964194 ((alk. paper))

LCCN

2016952805

OCLC

973232308

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