Cholesterol homeostasis : methods and protocols / edited by Ingrid C. Gelissen, Andrew J. Brown.

Format
Book
Language
English
Published/​Created
New York : Humana Press, [2017]
Description
xi, 300 pages : illustrations (some color) ; 26 cm

Availability

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    Details

    Subject(s)
    Editor
    Library of Congress genre(s)
    Series
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • Overview of cholesterol homeostasis / Ingrid C. Gelissen and Andrew J. Brown
    • Hybrid in silico/in vitro approaches for the identification of functional cholesterol-binding domains in membrane proteins / Coralie Di Scala and Jacques Fantini
    • Structural stringency of cholesterol for membrane protein function utilizing stereoisomers as novel tools : a review / Md. Jafurulla and Amitabha Chattopadhyay
    • Manipulating cholesterol status within cells / Winnie Luu, Ingrid C. Gelissen, and Andrew J. Brown
    • Assaying low-density-lipoprotein (LDL) uptake into cells / Anke Loregger, Jessica K. Nelson, and Noam Zelcer
    • Use of L-sIDOL transgenic mice as a murine model to study hypercholestorolemia and atherosclerosis / Eser J. Zerenturk and Anna C. Calkin
    • CRISPR/Cas9-mediated generation of Niemann-Pick C1 knockout cell line / Ximing Du, Ivan Lukmantara, and Hongyuan Yang
    • Quantitative measurement of cholesterol in cell populations using flow cytometry and fluorescent perfringolysin O* / Jian Li, Peter L. Lee, and Suzanne R. Pfeffer
    • Transport assays for sterol-binding proteins : stopped-flow fluorescence methods for investigating intracellular cholesterol transport mechanisms of NPC2 protein / Leslie A. McCauliff and Judith Storch
    • Synthesis and live-cell imaging of fluorescent sterols for analysis of intracellular cholesterol transport / Maciej Modzel, Frederik W. Lund, and Daniel Wüstner
    • Measurement of cholesterol transfer from lysosome to peroxisome using an in vitro reconstitution assay / Jie Luo, Ya-Cheng Liao, Jian Xiao, and Bao-Liang Song
    • Measurement of mitochondrial cholesterol import using a mitochondria-targeted CYP11A1 fusion construct / Barry E. Kennedy, Mark Charman, and Barbara Karten
    • Identifying sterol response elements within promoters of genes / Laura J. Sharpe and Andrew J. Brown
    • Membrane extraction of HMG CoA reductase as determined by susceptibility of lumenal epitope to in vitro protease digestion / Lindsey L. Morris and Russell A. DeBose-Boyd
    • Determining the topology of membrane-bound proteins using PEGylation / Vicky Howe and Andrew J. Brown
    • Measuring activity of cholesterol synthesis enzymes using gas chromatography/mass spectrometry / Anika V. Prabhu, Winnie Luu, and Andrew J. Brown
    • Sterol analysis by quantitative mass spectrometry / Andrew M. Jenner and Simon H.J. Brown
    • Measurement of rates of cholesterol and fatty acid synthesis in vivo using tritiated water / Adam M. Lopez, Jen-Chieh Chuang, and Stephen D. Turley
    • Methods for monitoring ABCA1-dependent sterol release / Yoshio Yamauchi, Shinji Yokoyama, and Ta-Yuan Chang
    • ABC-transporter mediated sterol export from cells using radiolabeled sterols / Alryel Yang and Ingrid C. Gelissen
    • Measurement of macrophage-specific in vivo reverse cholesterol transport in mice / Wendy Jessup, Maaike Kockx, and Leonard Kritharides.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781493968732 ((alk. paper))
    • 1493968734 ((alk. paper))
    LCCN
    2016963794
    OCLC
    964379112
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