Diagnostic Bacteriology : Methods and Protocols / edited by Kimberly A. Bishop-Lilly.

Format
Book
Language
English
Published/​Created
New York, NY Springer New York Imprint : Humana Press, 2017.
Description
1 online resource (XII, 264 pages 34 illustrations, 22 illustrations in color.) : online resource.

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    Summary note
    This volume provides a comprehensive collection of protocols on molecular diagnostics of bacteria that will suit the needs of molecular biologists, clinical laboratorians, and physician scientists alike. Chapters detail common bacterial pathogens, protocols that can be applied to diverse or even unknown pathogens, digital PCR, next generation sequencing, and bioinformatic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Diagnostic Bacteriology: Methods and Protocols delivers a wide range of assay types all on the cutting edge of diagnostic bacteriology.
    Bibliographic references
    Includes bibliographical references and index.
    Source of description
    Online resource; title from PDF title page (SpringerLink, viewed June 12, 2017).
    Contents
    • Whole-genome enrichment using RNA probes and sequencing of Chlamydia trachomatis directly from clinical samples / Amanda Claire Brown and Mette T. Christiansen
    • Characterization of sinus microbiota by 16S sequencing from swabs / Thad W. Vickery, Jennifer M. Kofonow, and Vijay R. Ramakrishnan
    • Molecular subtyping of Salmonella typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR) / Véronique Wuyts, Wesley Mattheus, Nancy H.C. Roosens, Kathleen Marchal, Sophie Bertrand, and Sigrid C.J. De Keersmaecker
    • Detection of Helicobacter pylori DNA in formalin-fixed paraffin-embedded gastric biopsies using laser microdissection and qPCR / María Fernanda Loayza Villa, Valeria Liliana Herrera Sevilla, and Nicolás Vivar-Diaz
    • Mycobacterial load assay / Stephen H. Gillespie, Wilber Sabiiti, and Katarina Oravcova
    • Defining diagnostic biomarkers using shotgun proteomics and MALDI-TOF mass spectrometry / Jean Armengaud
    • Detection and typing of "Candidatus Phytoplasma" spp. in host DNA extracts using oligonucleotide-coupled fluorescent microspheres / Edel Pérez-López, Christine Hammond, Chrystel Olivier, and Tim J. Dumonceaux
    • Detection of Helicobacter pylori in the gastric mucosa by fluorescence in vivo hybridization / Silvia Fontenete, Marina Leite, Ceu Figueiredo, Paul Cos, and Nuno F. Azevedo
    • Rapid antibiotic susceptibility testing for urinary tract infections / Anja Mezger, Mats Nilsson, and Dan I. Andersson
    • Detection and differentiation of lyme spirochetes and other tick-borne pathogens from blood using real-time PCR with molecular beacons / Samantha Schlachter, Kamfai Chan, Salvatore A.E. Marras, and Nikhat Parveen
    • Methods for real-time PCR-based diagnosis of Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia abortus infections in an opened molecular diagnostic platform / Onya Opota, René Brouillet, Gilbert Greub, and Katia Jaton
    • Real-time PCR to identify Staphylococci and assay for virulence from blood / Charles E. Okolie
    • Multiplex peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for diagnosis of bacterial vaginosis / Antonio Machado and Nuno Cerca
    • Closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis / Marcos D. Trangoni, Andrea K. Gioffré, and Silvio L. Cravero
    • Highly specific ligation-dependent microarray detection of single nucleotide polymorphisms / Noa Wolff and Ivan Barišicʹ
    • Multilocus sequence typing (MLST) for Cronobacter spp / Susan Joseph and Stephen Forsythe
    • Diagnostic bacteriology : raman spectroscopy / Rebecca L. Pavlicek, Nicole J. Crane, Meron Ghebremedhin, Katherine E. Cilwa, and Eric A. Elster.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781493970377
    • 1493970372
    OCLC
    1058716295
    Doi
    • 10.1007/978-1-4939-7037-7
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