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Germinal Centers : Methods and Protocols / edited by Dinis Pedro Calado.
Format
Book
Language
English
Published/Created
New York, NY Springer New York Imprint : Humana Press, 2017.
Description
1 online resource (XI, 338 pages 64 illustrations, 49 illustrations in color.) : online resource.
Availability
Available Online
Online Content
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
ReCAP - Remote Storage
QH506 .M45 1984 vol.1623
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Details
Subject(s)
Medicine
[Browse]
Immunology
[Browse]
Germinal centers
[Browse]
B cells
[Browse]
Editor
Calado, Dinis Pedro
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology ; 1623.
[More in this series]
Methods in Molecular Biology, 1064-3745 ; 1623
Summary note
This volume provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Germinal Centers: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Notes
Includes index.
Bibliographic references
Includes bibliographical references.
Source of description
Online resource; title from PDF title page (SpringerLink, viewed June 7, 2017).
Contents
Analysis of the germinal center reaction in tissue sections / David Dominguez-Sola and Giorgio Cattoretti
Detecting gene expression in lymphoid microenvironments by laser microdissection and quantitative RT-PCR / Yang Zhang, Laura Garcia-Ibanez, Geoffrey Brown, and Kai-Michael Toellner
Tracking plasma cell differentiation in living mice with two-photon microscopy / Carolin Ulbricht, Randall L. Lindquist, Laura Tech, and Anja E. Hauser
Microanatomical labeling of germinal center structures for flow cytometry using photoactivation / Johanne T. Jacobsen and Gabriel D. Victora
Fate mapping and transcript profiling of germinal center cells by two-photon photoconversion / Imogen Moran and Tri Giang Phan
Intravital microscopy of T-B cell interactions in germinal centers / Changming Shih and Hai Qi
Identifying follicular regulatory T cells by confocal microscopy / Ine Vanderleyden and Michelle A. Linterman
Cytokine expression by T follicular helper cells / Christoph Jandl, Claudia Loetsch, and Cecile King
Follicular dendritic cell isolation and loading of immune complexes / Balthasar A. Heesters, Cees E. Van der Poel, and Michael C. Carroll
Isolation and characterization of mouse and human follicular dendritic cells / Mohey Eldin M. El Shikh, Riham El Sayed, and Costantino Pitzalis
In vitro-induced germinal center B cell culture system / Kei Haniuda, Takuya Nojima, and Daisuke Kitamura
CRISPR/Cas9-mediated in vitro mutagenesis in GC-like B cells / Van Trung Chu, Robin Graf, and Klaus Rajewsky
Germinal center formation with retrovirally transduced B cells for determining the role of specific molecules in vivo / Rinako Nakagawa
Characterization of the B cell transcriptome bound by RNA-binding proteins with iCLIP / Manuel D. Dıaz-Munoz, Elisa Monzon-Casanova, and Martin Turner
Flow-cytometric method measuring B cell surface immunoglobulin avidity / Davide Angeletti, Gregory M. Frank, and Jonathan W. Yewdell
Somatic hypermutation and affinity maturation analysis using the 4-hydroxy-3-nitrophenyl-acetyl (NP) system / Nicole Heise and Ulf Klein
Targeting gene function in germinal center B cells : a practical approach / Valentina Petrocelli and Stefano Casola
Development of mouse model systems of germinal center lymphomas / Eleni Kabrani and Sandrine Sander
AID-Cre-ERT2 model : a tool for monitoring B cell immune responses and generating selective hybridomas / Simon Le Gallou, Takuya Nojima, Daisuke Kitamura, Jean-Claude Weill, and Claude-Agne`s Reynaud
Determining the origin of human germinal center B cell-derived malignancies / Marc Seifert and Ralf Kuppers
Tracking B-cell repertoires and clonal histories in normal and malignant lymphocytes / Nicola J. Weston-Bell, Graeme Cowan, and Surinder S. Sahota
How to simulate a germinal center / Philippe A. Robert, Ananya Rastogi, Sebastian C. Binder, and Michael Meyer-Hermann.
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Other format(s)
Also available in an electronic version.
ISBN
9781493970957
149397095X
OCLC
1058296644
Doi
10.1007/978-1-4939-7095-7
Statement on language in description
Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Germinal Centers [electronic resource] : Methods and Protocols / edited by Dinis Pedro Calado.
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99125108705906421