Princeton University Library Catalog

Heterologous gene expression in E. coli : methods and protocols / edited by Nicola A. Burgess-Brown.

New York : Humana Press, [2017]
xiv, 429 pages : illustrations (some color) ; 26 cm.
Bibliographic references:
Includes bibliographical references and index.
Recombinant protein expression in E. coli : a historical perspective / Opher Gileadi -- N- and C-terminal truncations to enhance protein solubility and crystallization : predicting protein domain boundaries with bioinformatics tools / Christopher D.O. Cooper and Brian D. Marsden -- Harnessing the profinity eXact system for expression and purification of heterologous proteins in E. coli / Yoav Peleg, Vadivel Prabahar, Dominika Bednarczyk, and Tamar Unger -- ESPRIT : a method for defining soluble expression constructs in poorly Understood Gene Sequences / Philippe J. Mas and Darren J. Hart -- Optimizing expression and solubility of proteins in E. coli using modified media and induction parameters / Troy Taylor, John-Paul Denson, and Dominic Esposito -- Optimization of membrane protein production using titratable strains of E. coli / Rosa Morra, Kate Young, David Casas-Mao, Neil Dixon, and Louise E. Bird -- Optimizing E. coli-based membrane protein production using Lemo21(DE3) or pReX and GFP-fusions / Grietje Kuipers, Markus Peschke, Nurzian Bernsel Ismail, Anna Hjelm, Susan Schlegel, David Vikström, Joen Luirink, and Jan-Willem de Gier -- High yield of recombinant protein in shaken E. coli cultures with enzymatic glucose release medium EnPresso B / Kaisa Ukkonen, Antje Neubauer, Vinit J. Pereira, and Antti Vasala -- Generic protocol for purifying disulfide-bonded domains and random protein fragments using fusion proteins with SUMO3 and cleavage by SenP2 protease / Hüseyin Besir -- Strategy for production of correctly folded disulfide-rich peptides in the periplasm of E. coli / Natalie J. Saez, Ben Cristofori-Armstrong, Raveendra Anangi, and Glenn F. King -- Split GFP complementation as reporter of membrane protein expression and stability in E. coli : a tool to engineer stability in a LAT transporter / Ekaitz Errasti-Murugarren, Arturo Rodríguez-Banqueri, and José Luis Vázquez-Ibar -- Acting on folding effectors to improve recombinant protein yields and functional quality / Ario de Marco -- Protein folding using a vortex fluidic device / Joshua Britton, Joshua N. Smith, Colin L. Raston, and Gregory A. Weiss -- Removal of affinity tags with TEV protease / Sreejith Raran-Kurussi, Scott Cherry, Di Zhang, and David S. Waugh -- Generation of recombinant n-linked glycoproteins in E. coli / Benjamin Strutton, Stephen R.P. Jaffé, Jagroop Pandhal, and Phillip C. Wright -- Production of protein kinases in E. coli / Charlotte A. Dodson -- Expression of prokaryotic integral membrane proteins in E. coli / James D. Love -- Multiprotein complex production in E. coli : the SecYEG-SecDFYajC-YidC holotranslocon / Imre Berger, Quiyang Jiang, Ryan J. Schulze, Ian Collinson, and Christiane Schaffitzel -- Membrane protein production in E. coli lysates in presence of preassembled nanodiscs / Ralf-Bernhardt Rues, Alexander Gräwe, Erik Henrich, and Frank Bernhard -- Not limited to E. coli : versatile expression vectors for mammalian protein expression / Katharina Karste, Maren Bleckmann, and Joop van den Heuvel -- Generic protocol for intracellular expression of recombinant proteins in Bacillus subtilis / Trang Phan, Phuong Huynh, Tuom Truong, and Hoang Nguyen -- In vivo biotinylation of antigens in E. coli / Susanne Gräslund, Pavel Savitsky, and Susanne Müller-Knapp -- Cold-shock expression system in E. coli for protein NMR studies / Toshihiko Sugiki, Toshimichi Fujiwara, and Chojiro Kojima -- High-throughput production of proteins in E. coli for structural studies / Charikleia Black, John J. Barker, Richard B. Hitchman, Hok Sau Kwong, Sam Festenstein, and Thomas B. Acton -- Mass spectrometric analysis of proteins / Rod Chalk -- How to determine interdependencies of glucose and lactose uptake rates for heterologous protein production with E. coli / David J. Wurm, Christoph Herwig, and Oliver Spadiut -- Interfacing biocompatible reactions with engineered Escherichia coli / Stephen Wallace and Emily P. Balskus.
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