Heterologous gene expression in E. coli : methods and protocols / edited by Nicola A. Burgess-Brown.

Format
Book
Language
English
Published/​Created
New York : Humana Press, [2017]
Description
xiv, 429 pages : illustrations (some color) ; 26 cm.

Details

Subject(s)
Editor
Library of Congress genre(s)
Series
Bibliographic references
Includes bibliographical references and index.
Contents
  • Recombinant protein expression in E. coli : a historical perspective / Opher Gileadi
  • N- and C-terminal truncations to enhance protein solubility and crystallization : predicting protein domain boundaries with bioinformatics tools / Christopher D.O. Cooper and Brian D. Marsden
  • Harnessing the profinity eXact system for expression and purification of heterologous proteins in E. coli / Yoav Peleg, Vadivel Prabahar, Dominika Bednarczyk, and Tamar Unger
  • ESPRIT : a method for defining soluble expression constructs in poorly Understood Gene Sequences / Philippe J. Mas and Darren J. Hart
  • Optimizing expression and solubility of proteins in E. coli using modified media and induction parameters / Troy Taylor, John-Paul Denson, and Dominic Esposito
  • Optimization of membrane protein production using titratable strains of E. coli / Rosa Morra, Kate Young, David Casas-Mao, Neil Dixon, and Louise E. Bird
  • Optimizing E. coli-based membrane protein production using Lemo21(DE3) or pReX and GFP-fusions / Grietje Kuipers, Markus Peschke, Nurzian Bernsel Ismail, Anna Hjelm, Susan Schlegel, David Vikström, Joen Luirink, and Jan-Willem de Gier
  • High yield of recombinant protein in shaken E. coli cultures with enzymatic glucose release medium EnPresso B / Kaisa Ukkonen, Antje Neubauer, Vinit J. Pereira, and Antti Vasala
  • Generic protocol for purifying disulfide-bonded domains and random protein fragments using fusion proteins with SUMO3 and cleavage by SenP2 protease / Hüseyin Besir
  • Strategy for production of correctly folded disulfide-rich peptides in the periplasm of E. coli / Natalie J. Saez, Ben Cristofori-Armstrong, Raveendra Anangi, and Glenn F. King
  • Split GFP complementation as reporter of membrane protein expression and stability in E. coli : a tool to engineer stability in a LAT transporter / Ekaitz Errasti-Murugarren, Arturo Rodríguez-Banqueri, and José Luis Vázquez-Ibar
  • Acting on folding effectors to improve recombinant protein yields and functional quality / Ario de Marco
  • Protein folding using a vortex fluidic device / Joshua Britton, Joshua N. Smith, Colin L. Raston, and Gregory A. Weiss
  • Removal of affinity tags with TEV protease / Sreejith Raran-Kurussi, Scott Cherry, Di Zhang, and David S. Waugh
  • Generation of recombinant n-linked glycoproteins in E. coli / Benjamin Strutton, Stephen R.P. Jaffé, Jagroop Pandhal, and Phillip C. Wright
  • Production of protein kinases in E. coli / Charlotte A. Dodson
  • Expression of prokaryotic integral membrane proteins in E. coli / James D. Love
  • Multiprotein complex production in E. coli : the SecYEG-SecDFYajC-YidC holotranslocon / Imre Berger, Quiyang Jiang, Ryan J. Schulze, Ian Collinson, and Christiane Schaffitzel
  • Membrane protein production in E. coli lysates in presence of preassembled nanodiscs / Ralf-Bernhardt Rues, Alexander Gräwe, Erik Henrich, and Frank Bernhard
  • Not limited to E. coli : versatile expression vectors for mammalian protein expression / Katharina Karste, Maren Bleckmann, and Joop van den Heuvel
  • Generic protocol for intracellular expression of recombinant proteins in Bacillus subtilis / Trang Phan, Phuong Huynh, Tuom Truong, and Hoang Nguyen
  • In vivo biotinylation of antigens in E. coli / Susanne Gräslund, Pavel Savitsky, and Susanne Müller-Knapp
  • Cold-shock expression system in E. coli for protein NMR studies / Toshihiko Sugiki, Toshimichi Fujiwara, and Chojiro Kojima
  • High-throughput production of proteins in E. coli for structural studies / Charikleia Black, John J. Barker, Richard B. Hitchman, Hok Sau Kwong, Sam Festenstein, and Thomas B. Acton
  • Mass spectrometric analysis of proteins / Rod Chalk
  • How to determine interdependencies of glucose and lactose uptake rates for heterologous protein production with E. coli / David J. Wurm, Christoph Herwig, and Oliver Spadiut
  • Interfacing biocompatible reactions with engineered Escherichia coli / Stephen Wallace and Emily P. Balskus.
Other format(s)
Also available in an electronic version.
ISBN
  • 1493968858
  • 9781493968855
LCCN
2017934051
OCLC
964653235
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