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Heterologous gene expression in E. coli : methods and protocols / edited by Nicola A. Burgess-Brown.
Format
Book
Language
English
Published/Created
New York : Humana Press, [2017]
Description
xiv, 429 pages : illustrations (some color) ; 26 cm.
Availability
Available Online
Online Content
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
ReCAP - Remote Storage
QH506 .M45 1984 vol.1586
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Details
Subject(s)
Escherichia coli
—
Genetics
—
Laboratory manuals
[Browse]
Gene expression
—
Laboratory manuals
[Browse]
Editor
Burgess-Brown, Nicola A.
[Browse]
Burgess-Brown, Nicola A.
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1586.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in Molecular Biology, 1064-3745 ; 1586
Springer protocols
Bibliographic references
Includes bibliographical references and index.
Contents
Recombinant protein expression in E. coli : a historical perspective / Opher Gileadi
N- and C-terminal truncations to enhance protein solubility and crystallization : predicting protein domain boundaries with bioinformatics tools / Christopher D.O. Cooper and Brian D. Marsden
Harnessing the profinity eXact system for expression and purification of heterologous proteins in E. coli / Yoav Peleg, Vadivel Prabahar, Dominika Bednarczyk, and Tamar Unger
ESPRIT : a method for defining soluble expression constructs in poorly Understood Gene Sequences / Philippe J. Mas and Darren J. Hart
Optimizing expression and solubility of proteins in E. coli using modified media and induction parameters / Troy Taylor, John-Paul Denson, and Dominic Esposito
Optimization of membrane protein production using titratable strains of E. coli / Rosa Morra, Kate Young, David Casas-Mao, Neil Dixon, and Louise E. Bird
Optimizing E. coli-based membrane protein production using Lemo21(DE3) or pReX and GFP-fusions / Grietje Kuipers, Markus Peschke, Nurzian Bernsel Ismail, Anna Hjelm, Susan Schlegel, David Vikström, Joen Luirink, and Jan-Willem de Gier
High yield of recombinant protein in shaken E. coli cultures with enzymatic glucose release medium EnPresso B / Kaisa Ukkonen, Antje Neubauer, Vinit J. Pereira, and Antti Vasala
Generic protocol for purifying disulfide-bonded domains and random protein fragments using fusion proteins with SUMO3 and cleavage by SenP2 protease / Hüseyin Besir
Strategy for production of correctly folded disulfide-rich peptides in the periplasm of E. coli / Natalie J. Saez, Ben Cristofori-Armstrong, Raveendra Anangi, and Glenn F. King
Split GFP complementation as reporter of membrane protein expression and stability in E. coli : a tool to engineer stability in a LAT transporter / Ekaitz Errasti-Murugarren, Arturo Rodríguez-Banqueri, and José Luis Vázquez-Ibar
Acting on folding effectors to improve recombinant protein yields and functional quality / Ario de Marco
Protein folding using a vortex fluidic device / Joshua Britton, Joshua N. Smith, Colin L. Raston, and Gregory A. Weiss
Removal of affinity tags with TEV protease / Sreejith Raran-Kurussi, Scott Cherry, Di Zhang, and David S. Waugh
Generation of recombinant n-linked glycoproteins in E. coli / Benjamin Strutton, Stephen R.P. Jaffé, Jagroop Pandhal, and Phillip C. Wright
Production of protein kinases in E. coli / Charlotte A. Dodson
Expression of prokaryotic integral membrane proteins in E. coli / James D. Love
Multiprotein complex production in E. coli : the SecYEG-SecDFYajC-YidC holotranslocon / Imre Berger, Quiyang Jiang, Ryan J. Schulze, Ian Collinson, and Christiane Schaffitzel
Membrane protein production in E. coli lysates in presence of preassembled nanodiscs / Ralf-Bernhardt Rues, Alexander Gräwe, Erik Henrich, and Frank Bernhard
Not limited to E. coli : versatile expression vectors for mammalian protein expression / Katharina Karste, Maren Bleckmann, and Joop van den Heuvel
Generic protocol for intracellular expression of recombinant proteins in Bacillus subtilis / Trang Phan, Phuong Huynh, Tuom Truong, and Hoang Nguyen
In vivo biotinylation of antigens in E. coli / Susanne Gräslund, Pavel Savitsky, and Susanne Müller-Knapp
Cold-shock expression system in E. coli for protein NMR studies / Toshihiko Sugiki, Toshimichi Fujiwara, and Chojiro Kojima
High-throughput production of proteins in E. coli for structural studies / Charikleia Black, John J. Barker, Richard B. Hitchman, Hok Sau Kwong, Sam Festenstein, and Thomas B. Acton
Mass spectrometric analysis of proteins / Rod Chalk
How to determine interdependencies of glucose and lactose uptake rates for heterologous protein production with E. coli / David J. Wurm, Christoph Herwig, and Oliver Spadiut
Interfacing biocompatible reactions with engineered Escherichia coli / Stephen Wallace and Emily P. Balskus.
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ISBN
1493968858
9781493968855
LCCN
2017934051
OCLC
964653235
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Heterologous Gene Expression in E.coli [electronic resource] : Methods and Protocols / edited by Nicola A. Burgess-Brown.
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99125108708006421