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The renin-angiotensin-aldosterone system : methods and protocols / edited by Sean E. Thatcher.
Uniform title
Renin-angiotensin-aldosterone system (Thatcher)
Format
Book
Language
English
Published/Created
New York, NY : Humana Press : Springer, [2017]
©2017
Description
x, 201 pages : illustrations (some color) ; 27 cm
Availability
Available Online
Online Content
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
ReCAP - Remote Storage
QH506 .M45 1984 vol.1614
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Details
Subject(s)
Renin-angiotensin system
—
Laboratory manuals
[Browse]
Renin-angiotensin system
[Browse]
Editor
Thatcher, Sean E.
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1614.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 1614
Springer protocols, 1949-2448
Summary note
This volume discusses protocols that aid in measuring different components in the renin-angiotensin-aldosterone system (RAAS). The book also looks at the methods used to assess angiotensin peptides and discerning the influence of RAAS components on different mammalian diseases. The chapters cover topics such as the use of fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta, blood pressure monitoring using the radio telemetry method, and the analysis of angiotensin metabolism in the kidney using mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and comprehensive, The Renin-Angiotensin-Aldosterone Systems: Methods and Protocols is a valuable resource that provides scientists and researchers with the best approaches to examine RAAS.
Bibliographic references
Includes bibliographical references and index.
Contents
Brief introduction into the Renin-Angiotensin-Aldosterone system : new and old techniques / Sean E. Thatcher
Color segmentation-based method to quantify atherosclerotic lesion compositions with immunostaining / Congqing Wu, Alan Daugherty, and Hong Lu
Assessment of protein carbonylation and protein tyrosine phosphatase (PTP) oxidation in vascular smooth muscle cells (VSMCs) using immunoblotting approaches / Sofia Tsiropoulou and Rhian M. Touyz
Methods for studying the role of RAAS in the modulation of vascular repair-relevant functions of stem/progenitor cells / Yagna P.R. Jarajapu
Use of a fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta / Yu Wang, Lisa A. Cassis, and Sean E. Thatcher
Measuring blood pressure using a noninvasive tail cuff method in mice / Yu Wang, Sean E. Thatcher, and Lisa A. Cassis
Blood pressure monitoring using radio telemetry method in mice / Yu Wang, Sean E. Thatcher, and Lisa A. Cassis
Characterization and functional phenotyping of renal immune cells via flow cytometry / Nathan P. Rudemiller and Steven D. Crowley
Assessment of the renin-angiotensin system in cellular organelle : new arenas for study in the mitochondria / Bryan A. Wilson and Mark C. Chappell
Comprehensive assessments of energy balance in mice / Justin L. Grobe
In vitro assays to determine smooth muscle cell hypertrophy, protein content, and fibrosis / Katherine J. Elliott and Satoru Eguchi
New mouse model for introduction of aortic aneurysm by implantation of deoxycorticosterone acetate pellets or aldosterone infusion in the presence of high salt / Shu Liu, Ming C. Gong, and Zhenheng Guo
Fluorescence-based binding assay for screening ligands of angiotensin receptors / Maiia E. Bragina, Nikolaos Stergiopulos, and Rodrigo A. Fraga-Silva
Primer to angiotensin peptide isolation, stability, and analysis by nano-liquid chromatography with mass detection / Mariola Olkowicz, Stefan Chlopicki, and Ryszard T. Smolenski
Analysis of angiotensin metabolism in the kidney using mass spectrometry / Nadja Grobe and Khalid M. Elased.
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Other format(s)
Also available in an electronic version.
ISBN
9781493970285 ((alk. paper))
1493970283 ((alk. paper))
LCCN
2017937363
OCLC
973920026
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The Renin-Angiotensin-Aldosterone System [electronic resource] : Methods and Protocols / edited by Sean E. Thatcher.
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99125108706706421