Bacterial protein secretion systems : methods and protocols / editors Laure Journet and Eric Cascales.

Format
Book
Language
English
Published/​Created
New York, NY : Humana Press ; Springer, c2017.
Description
xv, 528 pages : illustrations (some color).

Availability

Copies in the Library

Location Call Number Status Location Service Notes
ReCAP - Remote StorageQH506 M45 1984 vol.1615 Browse related items Request

    Details

    Subject(s)
    Editor
    Library of Congress genre(s)
    Series
    • Methods in molecular biology (Clifton, N.J.) ; v. 1615. [More in this series]
    • Methods in molecular biology, 1064-3745 ; v. 1615
    Bibliographic references
    Includes bibliographical references and index.
    Source of description
    Online resource; title from PDF title page (SpringerProtocol, viewed July 5, 2017).
    Contents
    • Identification of protein secretion systems in bacterial genomes using MacSyFinder / Sophie S. Abby and Eduardo P.C. Rocha
    • Protein sorting prediction / Henrik Nielsen
    • Cell fractionation / Melissa Petiti, Laetitia Houot, and Denis Duché
    • Defining lipoprotein localisation by fluorescence microscopy / Maria Guillermina Casabona, Mylène Robert-Genthon, Didier Grunwald, and Ina Attrée
    • Identification of lipoproteins using globomycin and radioactive palmitate / Nienke Buddelmeijer
    • Defining membrane protein localization by isopycnic density gradients / Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow
    • Cell surface exposure / Anna Konovalova
    • Probing inner membrane protein topology by proteolysis / Maxence S. Vincent and Eric Cascales
    • Mapping of membrane protein topology by substituted cysteine accessibility method (SCAM) / Mikhail Bogdanov
    • Defining membrane protein topology using pho-lac reporter fusions / Gouzel Karimova and Daniel Ladant
    • In vivo and in vitro protein-peptidoglycan interactions / Gang Li and S. Peter Howard
    • Measure of peptidoglycan hydrolase activity / Yoann G. Santin and Eric Cascales
    • Protein-protein interaction : Bacterial Two-Hybrid / Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant
    • Protein-protein interactions : yeast two-hybrid system / Jer-Sheng Lin and Erh-Min Lai
    • Protein-protein interactions : cytology two-hybrid / Krishnamohan Atmakuri
    • Fusion reporter approaches to monitoring transmembrane helix interactions in bacterial membranes / Laureen Logger, Abdelrahim Zoued, and Eric Cascales
    • Protein-protein interactions : co-immunoprecipitation / Jer-Sheng Lin and Erh-Min Lai
    • Protein-protein interaction : tandem affinity purification in bacteria / Julie P.M. Viala and Emmanuelle Bouveret
    • Site-directed and time-resolved photocrosslinking in cells metabolically labeled with radioisotopes / Raffaele Ieva
    • Protein-protein interactions : pull-down assays / Arthur Louche, Suzana P. Salcedo, and Sarah Bigot
    • Protein-protein interactions : surface plasmon resonance / Badreddine Douzi
    • Assessing energy-dependent protein conformational changes in the TonB system / Ray A. Larsen
    • Defining assembly pathways by fluorescence microscopy / Abdelrahim Zoued and Andreas Diepold
    • Large complexes : cloning strategy, production, and purification / Eric Durand and Roland Lloubes
    • Shearing and enrichment of extracellular type IV pili / Alba Katiria Gonzalez Rivera and Katrina T. Forest
    • Blue native PAGE analysis of bacterial secretion complexes / Susann Zilkenat, Tobias Dietsche, Julia V. Monjarás Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner
    • In situ imaging of bacterial secretion systems by electron cryotomography / Gregor L. Weiss, João M. Medeiros, and Martin Pilhofer
    • Structural analysis of protein complexes by cryo electron microscopy / Tiago R.D. Costa, Athanasios Ignatiou, and Elena V. Orlova
    • Bacterial filamentous appendages investigated by solid-state NMR spectroscopy / Birgit Habenstein and Antoine Loquet
    • Energy requirements for protein secretion via the flagellar type III secretion system / Marc Erhardt
    • Identification of effectors : precipitation of supernatant material / Nicolas Flaugnatti and Laure Journet
    • Screening for secretion of the type VI secretion system protein Hcp by enzyme-linked immunosorbent assay and colony blot / Brent S. Weber, Pek Man Ly, and Mario F. Feldman
    • Effector translocation : cya reporter assay / Suma Chakravarthy, Bethany Huot, and Brian H. Kvitko
    • Monitoring effector translocation using the TEM-1 beta-lactamase reporter system / Julie Allombert, Anne Vianney, and Xavier Charpentier
    • Effector translocation assay : differential solubilization / Irina S. Franco, Sara V. Pais, Nuno Charro, and Luís Jaime Mota
    • Quantitative determination of anti-bacterial activity during bacterial co-culture / Juliana Alcoforado Diniz, Birte Hollmann, and Sarah J. Coulthurst.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781493970339 ((electronic bk.))
    • 149397033X ((electronic bk.))
    OCLC
    1171465708
    Statement on language in description
    Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage. Read more...
    Other views
    Staff view