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Alpha-1 antitrypsin deficiency : methods and protocols / edited by Florie Borel, Christian Mueller.
Format
Book
Language
English
Published/Created
New York : Humana Press ; Springer, [2017]
©2017
Description
xiii, 278 pages : illustrations (some color) ; 26 cm
Availability
Available Online
Online Content
Springer Nature - Complete Protocols
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Location Service
Notes
ReCAP - Remote Storage
QH506 .M45 1984 vol.1639
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Details
Subject(s)
Alpha 1-antitrypsin deficiency
—
Laboratory manuals
[Browse]
Editor
Borel, Florie
[Browse]
Mueller, Christian (Gene therapist)
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Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 1639.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; volume no. 1639
Springer protocols, 1949-2448
Summary note
This volume provides protocols that expand on the latest alpha-1 antitrypsin (AAT) research. The chapters in this book are divided in to three sections: Part I is dedicated to patient-oriented research; part II discusses animal models; and Part III focuses on in vitro studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Alpha-1 Antitrypsin Deficiency: Methods and Protocols is a valuable resource for researchers, students, and clinican-scientists interested in AAT deficiency, as well as anyone working in the fields of pulmonology and hepatology.
Bibliographic references
Includes bibliographical references and index.
Contents
Pathophysiology of alpha-1 antitrypsin deficiency liver disease / Jeffrey H. Teckman and Keith S. Blomenkamp
Pathophysiology of alpha-1 antitrypsin lung disease / Michael Kalfopoulos, Kaitlyn Wetmore, and Mai K. ElMallah
Measuring and interpreting serum AAT concentration / Leslie J. Donato, Melissa R. Snyder, and Dina N. Greene
AAT phenotype identification by isoelectric focusing / Dina N. Greene, M.C. Elliott-Jelf, and David G. Grenache
Laboratory diagnosis by genotyping / Irene Belmonte, Luciana Montoto, and Francisco Rodrıguez-Frıas
Genotyping protocol for the alpha-1 antitrypsin (PiZ) mouse model / Alisha M. Gruntman
Elastase-induced lung emphysema models in mice / Bela Suki, Erzsebet Bartolak-Suki, and Patricia R.M. Rocco
Assessing structure-function relations in mice using the forced oscillation technique and quantitative histology / Harikrishnan Parameswaran and Bela Suki
Practical methods for assessing emphysema severity based on estimation of linear mean intercept (Lm) in the context of animal models of alpha-1 antitrypsin deficiency / Airiel M. Davis, Kristen E. Thane, and Andrew M. Hoffman
Design, cloning, and in vitro screening of artificial miRNAs to silence alpha-1 antitrypsin / Florie Borel and Christian Mueller
Methods to identify and characterize siRNAs targeting serpin A1 in vitro and in vivo using RNA interference / Stuart Milstein, Kun Qian, Linying Zhang, and Alfica Sehgal
Knockdown of Z mutant alpha-1 antitrypsin in vivo using modified DNA antisense oligonucleotides / Mariam Aghajan, Shuling Guo, and Brett P. Monia
Immunohistochemistry staining for human alpha-1 antitrypsin / Dongtao A. Fu and Martha Campbell-Thompson-- Periodic acid-schiff staining with diastase / Dongtao A. Fu and Martha Campbell-Thompson
Protocol for directed differentiation of human induced pluripotent stem cells (iPSCs) to a hepatic lineage / Joseph E. Kaserman and Andrew A. Wilson
Isolation of kupffer cells and hepatocytes from a single mouse liver / Marcela Aparicio-Vergara, Michaela Tencerova, Cecilia Morgantini, Emelie Barreby, and Myriam Aouadi
Alpha-1 antitrypsin transcytosis and secretion / Angelia D. Lockett
Measuring the effect of histone deacetylase inhibitors (HDACi) on the secretion and activity of alpha-1 antitrypsin / Chao Wang, Marion Bouchecareilh, and William E. Balch
Expression and purification of active recombinant human Alpha-1 antitrypsin (AAT) from Escherichia coli / Beena Krishnan, Lizbeth Hedstrom, Daniel N. Hebert, Lila M. Gierasch, and Anne Gershenson
Quantification of total human alpha-1 antitrypsin by sandwich ELISA / Qiushi Tang, Alisha M. Gruntman, and Terence R. Flotte
Quantification of murine AAT by direct ELISA / Andrew Cox and Christian Mueller
Quantification of Z-AAT by a Z-specific "sandwich" ELISA / Florie Borel, Qiushi Tang, and Christian Mueller
Semiquantitation of monomer and polymer alpha-1 antitrypsin by centrifugal separation and assay by western blot of soluble and insoluble components / Keith S. Blomenkamp and Jeffrey H. Teckman
Electrophoresis- and FRET-based measures of serpin polymerization / Sarah V. Faull, Anwen E. Brown, Imran Haq, and James A. Irving
Therapeutics : alpha-1 antitrypsin augmentation therapy / Michael Campos and Jorge Lascano
Therapeutic options in alpha-1 antitrypsin deficiency : liver transplantation / Nedim Hadzic
Therapeutics : gene therapy for alpha-1 antitrypsin deficiency / Alisha M. Gruntman and Terence R. Flotte.
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Also available in an electronic version.
ISBN
9781493971619
1493971611
LCCN
2017943681
OCLC
982651663
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Alpha-1 Antitrypsin Deficiency [electronic resource] : Methods and Protocols / edited by Florie Borel, Christian Mueller.
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99125108704406421