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Membrane protein structure and function characterization : methods and protocols / edited by Jean-Jacques Lacapere.
Format
Book
Language
English
Published/Created
New York, NY : Humana Press, [2017]
Description
xii, 419 pages : illustrations (some color) ; 26 cm
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
ReCAP - Remote Storage
QH506 .M45 1984 vol.1635
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Details
Subject(s)
Membrane proteins
—
Laboratory manuals
[Browse]
Membrane proteins
—
Structure
[Browse]
Editor
Lacapère, Jean-Jacques
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1635.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 1635
Springer protocols
Summary note
In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Bibliographic references
Includes bibliographical references and index.
Contents
Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2
Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex
Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris protoplasts
Cell-free Expression for the Study of Hydrophobic Proteins: The Example of Yeast ATP-synthase subunits
Wheat Germ Cell Free Overexpression for the Production of Membrane Proteins
Methyl-specific Isotope Labelling Strategies for NMR Studies of Membrane Proteins
Labeling of Membrane Complexes for Electron Microscopy
Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins Case Study of The Mouse 5-HT Receptor
Characterization of New Detergent and Detergent Mimetics by Scattering Techniques for Membrane Protein Crystallization
Secondary Structure Determination by Means of ATR-FTIR Spectroscopy
Native Mass Spectroscopy for the Characterization of Structure and Interactions of Membrane Proteins
Mass Spectroscopy of Mitochondrial Membrane Proteins Complexes
Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+NQR from V cholerae in the Presence of Lipids
Reconstitution of Membrane Protein in Liposomes
Ion Channels as Reporters of Membrane Receptor Function: Automated Analysis in Xenopus Oocytes
The CRACAM Robot: Two Dimensional Crystallization of Membrane Protein
Reconstitution of Membrane Proteins into Nanodiscs for Single Particle Electron Microscopy
Solid-state NMR of Membrane Protein Reconstituted in Proteoliposomes, the Case of TSPO
Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR
Simulation of Ligand Binding To Membrane Proteins
Molecular modelling of Transporters: From Low Resolution Cryo-Electron Microscopy Map to Conformational Exploration. The Example of TSPO Molecular Dynamic of Membrane Protein in Different Environments.
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Other format(s)
Also available in an electronic version.
ISBN
9781493971497 ((alk. paper))
1493971492
LCCN
2017943682
OCLC
981117834
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Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Membrane Protein Structure and Function Characterization [electronic resource] : Methods and Protocols / edited by Jean-Jacques Lacapere.
id
99125108704806421