Urothelial Carcinoma : Methods and Protocols / edited by Wolfgang A Schulz, Mich謥 J. Hoffmann, G#xED25 ; r Niegisch.

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Format
Book
Language
English
Published/​Created
New York, NY : Springer New York : Imprint : Humana Press, 2018.
Description
1 online resource (XIV, 362 pages 73 illustrations, 49 illustrations in color.) : online resource

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    Summary note
    This volume details standard techniques for the characterization of urothelial carcinoma as well as methods to investigate mechanisms of carcinogenesis.èapters guide readers on cellular and animal models for urothelial carcinoma and related diseases, molecular analyses from body fluids, and new approaches to therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Urothelial Carcinoma: Methods and Protocols hopes that the techniques described in this volume will contribute to the current upturn in research on urothelial carcinoma and to the application of its results in clinical practice.
    Bibliographic references
    Includes bibliographical references and index.
    Source of description
    Online resource; title from PDF title page (SpringerProtocols, viewed September 11, 2017).
    Contents
    • Analysis of chromosomal alterations in urothelial carcinoma / Donatella Conconi and Angela Bentivegna
    • Analysis of point mutations in clinical samples of urothelial carcinoma / Mustafa Alamyar and Ellen C. Zwarthoff
    • Versatile assay for detection of aberrant DNA methylation in bladder Cancer / Stella Tommasi and Ahmad Besaratinia
    • Immunohistochemical analysis of urothelial carcinoma tissues for proliferation and differentiation markers / Michael Rose and Nadine T. Gaisa
    • Molecular subtype profiling of urothelial carcinoma using a subtype-specific immunohistochemistry panel / Gottfrid Sjodahl
    • Defining the pathways of urogenital schistosomiasis-sssociated urothelial carcinogenesis through transgenic and bladder wall egg injection models / Evaristus C. Mbanefo and Michael H. Hsieh
    • Algorithm for the automated evaluation of NAT2 genotypes / Georg Michael, Ricarda Thier, Meinolf Blaszkewicz, Silvia Selinski, and Klaus Golka
    • Detection of APOBEC3 proteins and catalytic activity in urothelial carcinoma / Ananda Ayyappan Jaguva Vasudevan, Wolfgang Goering, Dieter Haussinger, and Carsten Munk
    • Oxidative stress in urothelial carcinogenesis : measurements of protein carbonylation and intracellular production of reactive oxygen species / Patcharawalai Whongsiri, Suchittra Phoyen, and Chanchai Boonla
    • Urothelial carcinoma stem cells : current concepts, controversies, and methods / Jiri Hatina, Hamendra Singh Parmar, Michaela Kripnerova, Anastasia Hepburn, and Rakesh Heer
    • In vitro differentiation and propagation of urothelium from pluripotent stem cell lines / Stephanie L. Osborn and Eric A. Kurzrock
    • Spheroid cultures of primary urothelial cancer cells : cancer tissue-originated spheroid (CTOS) method / Takahiro Yoshida, Hiroaki Okuyama, Hiroko Endo, and Masahiro Inoue
    • N-butyl-N-4-hydroxybutyl nitrosamine mouse urinary bladder cancer model / Paula A. Oliveira, Carmen Vasconcelos-Nobrega, Rui M. Gil da Costa, and Regina Arantes-Rodrigues
    • Patient-derived bladder cancer xenografts / Carina Bernardo and Lucio Lara Santos
    • Orthotopic mouse models of urothelial cancer / Wolfgang Jager, Igor Moskalev, Peter Raven, Akihiro Goriki, Samir Bidnur, and Peter C. Black
    • Quantification of microRNAs in urine-derived specimens / Susanne Fuessel, Andrea Lohse-Fischer, Dana Vu Van, Karsten Salomo, Kati Erdmann, and Manfred P. Wirth
    • Quantitative RNA analysis from urine using real time PCR / Lourdes Mengual and Mireia Olivan
    • DNA methylation analysis from body fluids / Dimo Dietrich
    • Urinary protein markers for the detection and prognostication of urothelial carcinoma / Tibor Szarvas, Peter Nyirady, Takashi Kobayashi, Osamu Ogawa, Charles J. Rosser, and Hideki Furuya
    • Isolation and characterization of CTCs from patients with cancer of a urothelial origin / Vladimir Bobek and Katarina Kolostova.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781493972340
    • 1493972340
    • 9781493972333 ((alk. paper))
    • 1493972332
    OCLC
    1021189750
    Doi
    • 10.1007/978-1-4939-7234-0
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