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Extracellular vesicles : methods and protocols / edited by Winston Patrick Kuo, Shidong Jia.
Format
Book
Language
English
Published/Created
New York : Humana Press ; Springer, [2017]
©2017
Description
xvi, 446 pages : illustrations (some color) ; 26 cm.
Availability
Available Online
SpringerNature Complete Protocols
Copies in the Library
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Call Number
Status
Location Service
Notes
ReCAP - Remote Storage
QH506 .M45 1984 vol.1660
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Details
Subject(s)
Ribonucleases
—
Laboratory manuals
[Browse]
Cell membranes
—
Formation
[Browse]
Editor
Kuo, Winston Patrick
[Browse]
Jia, Shidong
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Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 1660.
[More in this series]
Methods in Molecular Biology, 1064-3745 ; volume 1660
Summary note
This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Extracellular Vesicles: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications.
Bibliographic references
Includes bibliographical references and index.
Contents
Extracellular vesicles : a brief overview and its role in precision medicine / Mingyi Shang, John S. Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo, and Hongjun Kang
Red blood cells : a source of extracellular vesicles / Winston Patrick Kuo, John C. Tigges, Vasilis Toxavidis, and Ionita Ghiran
Isolation of extracellular vesicles by ultracentrifugation / Fatemeh Momen-Heravi
Sequential filtration : a gentle method for the isolation of functional extracellular vesicles / Mitja L. Heinemann and Jody Vykoukal
Paper-based for isolation of extracellular vesicles / Yi-Hsing Hsiao and Chihchen Chen
Filter-based extracellular vesicle mRNA isolation and high-throughput gene expression analysis / Cindy M. Yamamoto, Taku Murakami, and Shu-Wing Ng
Specific and generic isolation of extracellular vesicles with magnetic beads / Ketil W. Pedersen, Bente Kierulf, and Axl Neurauter
Polymer-based purification of extracellular vesicles / Peter N. Brown and Hang Yin
Size exclusion chromatography : a simple and reliable method for exosome purification / Richard Lobb and Andreas Moller
Purification protocols for extracellular vesicles / Rebecca E. Lane, Darren Korbie, Matt Trau, and Michelle M. Hill
Characterization of extracellular vesicles by surface plasmon resonance / Hyungsoon Im, Katherine Yang, Hakho Lee, and Cesar M. Castro
Extracellular vesicle isolation and analysis by western blotting / Emma J.K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, and George M. Church
Analysis of extracellular vesicles using fluorescence nanoparticle tracking analysis / Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, and Rebecca Dragovic
Characterization of extracellular vesicles by flow cytometry / Virginia Camacho, Vasilis Toxavidis, and John C. Tigges
Characterization of extracellular vesicles by size-exclusion high-performance liquid chromatography (HPLC) / Tao Huang and Jiang He
Multi-surface antigen staining of larger extracellular vesicles / Veronika Lukacs-Kornek, Henrike Julich-Haertel, Sabine Katharina Urban, and Miroslaw Kornek
Microcapillary chip-based extracellular vesicle profiling system / Takanori Akagi and Takanori Ichiki
Detection and characterization of extracellular vesicles by transmission and cryo-transmission electron microscopy / Petr Cizmar and Yuana Yuana
Imaging of isolated extracellular vesicles using fluorescence microscopy / Dmitry Ter-Ovanesyan, Emma J.K. Kowal, Aviv Regev, George M. Church, and Emanuele Cocucci
In vivo tracking of extracellular vesicles in mice using fusion protein comprising lactadherin and Gaussia Luciferase / Yuki Takahashi, Makiya Nishikawa, and Yoshinobu Takakura
Tracking extracellular vesicles delivery and RNA translation using multiplexed reporters / Anthony Yan-Tang Wu and Charles Pin-Kuang Lai
Extraction and analysis of extracellular vesicle-associated miRNAs following antibody-based extracellular vesicle capture from plasma samples / Davide Zocco and Natasa Zarovni
Extracellular vesicle miRNA detection using molecular beacons / Won Jong Rhee and Seunga Jeong
Rapid isolation of extracellular vesicles from blood plasma with size-exclusion chromatography followed by mass spectrometry-based proteomic profiling / Simion Kreimer and Alexander R. Ivanov
Adaptable polyethylene glycol-based workflow for proteomic analysis of extracellar vesicles / Stephanie N. Hurwitz and David G. Meckes Jr.
Protocol for exosome isolation from small volume of ovarian follicular fluid : evaluation of ultracentrifugation and commercial kits / Shlomit Kenigsberg, Brandon A. Wyse, Clifford L. Librach, and Juliano C. da Silveira
Isolation of extracellular vesicles in saliva using density gradient ultracentrifugation / Kazuya Iwai, Satoshi Yamamoto, Mitsutaka Yoshida, and Kiyotaka Shiba
Isolation of extracellular vesicles from breast milk / Xin Wang
Integrated double-filtration microfluidic device for detection of extracellular vesicles from urine for bladder cancer diagnosis / Li-Guo Liang, Ye-Feng Sheng, Sherry Zhou, Fatih Inci, Lanjuan Li, Utkan Demirci, and ShuQi Wang
Electric field-induced disruption and releasing viable content from extracellular vesicles / Chris Wang, Austin Wang, Fang Wei, David T.W. Wong, and Michael Tu
Production and characterization of extracellular vesicles in malaria / Smart Mbagwu, Michael Walch, Luis Filgueira, and Pierre-Yves Mantel
Isolation of extracellular vesicles from stem cells / Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma, Yutao Liu, Il-man Kim, Neal L. Weintraub, and Yaoliang Tang
Use of peripheral extracellular vesicles for identification of molecular biomarkers in a solid tumor mouse model / Noemı Garcia-Romero, Gorjana Rackov, Cristobal Belda-Iniesta, and Angel Ayuso-Sacido
Therapeutic applications of extracellular vesicles : perspectives from newborn medicine / Gareth R. Willis, Stella Kourembanas, and S. Alex Mitsialis
Therapeutic use of tumor cell-derived extracellular vesicles / Jing Liu, Jingwei Ma, Ke Tang, and Bo Huang.
Show 32 more Contents items
ISBN
1493972510
9781493972517
OCLC
988282193
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Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Extracellular Vesicles [electronic resource] : Methods and Protocols / edited by Winston Patrick Kuo, Shidong Jia.
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