Genes & signals / Mark Ptashne, Alexander Gann.

Author
Ptashne, Mark [Browse]
Format
Book
Language
English
Published/​Created
Cold Spring Harbor, New York : Cold Spring Harbor Laboratory Press, ©2002.
Description
xvi, 192 pages : illustrations ; 24 cm

Availability

Copies in the Library

Location Call Number Status Location Service Notes
Lewis Library - Stacks QH450 .P83 2002 Browse related items Request
    Lewis Library - Stacks QH450 .P83 2002 Browse related items Request

      Details

      Subject(s)
      Summary note
      P. 103.
      Bibliographic references
      Includes bibliographical references and index.
      Contents
      • Chapter 1 Lessons from Bacteria 11
      • Rna Polymerase 11
      • Regulated Recruitment: The lac Genes 13
      • Protein-DNA Interactions 15
      • Detecting Physiological Signals 16
      • Promoter Recognition and Transcription by RNA Polymerase 17
      • Switching the Genes On: Activation by CAP 18
      • Cooperative Binding of Proteins to DNA 18
      • Cooperative Binding and Gene Activation by CAP 21
      • Repression by Lac Repressor 25
      • Interim Summary and Extension 25
      • More Regulated Recruitment: The Bacteriophage [lambda] 26
      • The Switch 28
      • Establishing Lysogeny 29
      • Analogies with lac 31
      • Promoters 32
      • Protein-DNA Interactions 32
      • Repression 32
      • Activation 32
      • Detecting Physiological Signals 33
      • Making an Efficient Switch 34
      • Cooperative Binding of [lambda] Repressor to DNA 34
      • Autogenous Control by Repressor 37
      • Panel: Antitermination: N and Q 38
      • Interim Summary and Extensions 39
      • Activation: A Closer Look 39
      • DNA binding: A Closer Look 41
      • Synergy 41
      • Polymerase Activation: glnA and Related Genes 42
      • Promoter Recognition by [sigma superscript 54]-containing Polymerase 43
      • DNA Binding by NtrC 43
      • Detecting the Physiological Signal 43
      • Activation by NtrC 43
      • Other Activators of [sigma superscript 54] Polymerase 45
      • Interim Summary 45
      • Panel: Phage T4 Late Genes 47
      • Promoter Activation: merT and Related Genes 47
      • Promoter Recognition 47
      • Detecting the Physiological Signal and Activation 48
      • Interim Summary 48
      • Panel: More on Repression in Bacteria 51
      • Chapter 2 Yeast: A Single-celled Eukaryote 59
      • Rna Polymerase 60
      • Other Parts of the Transcriptional Machinery 60
      • An Overview of Activation 63
      • A Model Case: The Gal Genes 63
      • Overview of Regulation of a GAL Gene 64
      • Specific DNA Binding 65
      • Detecting Physiological Signals 66
      • How Gal4 Works 67
      • Separate DNA-binding and Activating Regions of Gal4 67
      • Activating Region Structure 71
      • Independent Mutations Can Increase Activator Strength Additively When Combined 72
      • Activating Regions Work with an Efficiency Proportional to Their Lengths 72
      • Activating Region-Target Interactions Tolerate Sequence Changes 73
      • New Activating Regions Are Easily Generated 73
      • Squelching 75
      • Recruitment Visualized 76
      • Activator Bypass Experiments 76
      • Activation by a Heterologous Protein-Protein Interaction 78
      • Direct Tethering of the Transcriptional Machinery 80
      • Panel: Activator Bypass Experiments 81
      • How does Gal4 Recruit Polymerase? 82
      • Nucleosomes and Their Modifiers 83
      • Targets of Gal4: Experiments Performed In Vitro 84
      • Action of Gal4: Experiments Performed In Vivo 85
      • What Is Required for Activation? 86
      • What Appears at the Gene Upon Activation? 86
      • Imposing a Need for Nucleosome Modifiers 87
      • Weakening the Gal4-binding Sites or the Gal4-activating region 87
      • Cell-cycle Stage-dependent Requirement for Nucleosome Modifiers 89
      • Activation: An Interim Summary 89
      • Repression by Mig1 91
      • Signal Integration and Combinatorial Control 92
      • Cooperative Binding with Alternative Partners 93
      • Sequential Binding of Activators 94
      • Silencing 95
      • Heterochromatic Chromosomal Regions 95
      • Compartmentalization 98
      • Panel: Variegation Visualized 99
      • Panel: Epigenetics 100
      • DNA Looping 101
      • Chapter 3 Some Notes on Higher Eukaryotes 115
      • Mechanism of Activation: Recruitment 116
      • What is Recruited? 118
      • Transcriptional Machinery and Promoters 118
      • Nucleosomal Templates 119
      • Targets 119
      • The Human Interferon-[beta] Gene 121
      • The Drosophila HSP70 Gene 122
      • Repression 122
      • Detecting and Transmitting Physiological Signals 123
      • Transport Into and Out of the Nucleus 123
      • Phosphorylation of Inhibitor or Activator in the Nucleus 124
      • Panel: Transport-dependent Proteolysis: The Case of SREBP 125
      • Allosteric Change of DNA-bound Activator 126
      • Signal Integration, Combinatorial Control, and Alternative Enhancers 126
      • The Human Interferon-[beta] Enhancer 126
      • The Drosophila eve Gene 128
      • Action at a Distance 129
      • Dna Methylation, Insulators, and Imprinting 132
      • Chromosomal Position and Gene Expression 134
      • Compartmentalization 135
      • Chapter 4 Enzyme Specificity and Regulation 143
      • Ubiquitylation and Proteolysis 144
      • Splicing 146
      • Imposing Specificity on Kinases 148
      • Cyclin-dependent Kinases 149
      • Transcriptional Antitermination by TAT 150
      • Cytokine Receptors and Signaling through STATs 152
      • Growth Factor Receptors 154
      • Interim Summary and Extensions 156
      • Kinases 157
      • Phosphatases 158
      • Interpreting Signals 159
      • Further Generalizations 160
      • Dangers 160
      • Interpreting Experiments 162
      • Benefits 163
      • Appendix 1 More on Cooperativity 177
      • Appendix 2 Topogenic Sequences 183
      • Appendix 3 All-or-none Effects and Levels of Gene Expression 185.
      Other title(s)
      Genes and signals
      ISBN
      • 0879696311 ((cloth))
      • 9780879696313 ((cloth))
      • 0879696338 ((pbk.))
      • 9780879696337 ((pbk.))
      LCCN
      2001053889
      OCLC
      48132093
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