Antibody engineering : methods and protocols / edited by Benny K.C. Lo.

Format
Book
Language
English
Published/​Created
Totowa, N.J. : Humana Press, ©2004.
Description
xiv, 561 pages : illustrations ; 24 cm.

Availability

Copies in the Library

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Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.248 Browse related items Request

    Details

    Subject(s)
    Library of Congress genre(s)
    Series
    • Methods in molecular biology (Clifton, N.J.) ; v. 248. [More in this series]
    • Methods in molecular biology ; 248
    Summary note
    With 10 recent FDA approvals of therapeutic antibody drugs and hundreds more in the biotechnology pipeline, it is clear that engineered antibodies will play a major role in treating human diseases in the 21st century. In Antibody Engineering: Methods and Protocols, leading researchers and clinicians present a core collection of cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the generation of human recombinant antibodies by humanization approaches, molecular display technologies, and transgenic animals. The techniques for antibody expression and optimization utilize not only bacterial and mammalian cell cultures, but also insect cell cultures and transgenic plants. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics. Each fully tested protocol is described in step-by-step detail and includes background material, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Comprehensive and up-to-date, Antibody Engineering: Methods and Protocols provides both laboratory and clinical investigators with a diverse array of the fundamental techniques upon which new and imaginative drug technologies can be successfully developed.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • pt. 1. Antibody sequence and structure
    • Internet resources for the antibody engineer / Benny K.C. Lo
    • The Kabat database and a bioinformatics example / George Johnson
    • IMGT, the International ImMunoGeneTics Information Sysmtem, http://imgt.cines.fr / Marie-Paule Lefranc
    • Antibody variable regions: toward a unified modeling method / Nicholas Whitelegg
    • Studying antibody conformations by ultracentrifugation and hydrodynamic modeling / Stephen E. Harding
    • pt. 2. Antibody-lead generation
    • PCR cloning of human immunoglobulin genes / James D. Marks
    • Antibody humanization by CDR grafting / Benny K.C. Lo
    • Selection of human antibodies from phage display libraries / James D. Marks
    • Production of human single-chain antibodies by ribosome display / Mingyue He
    • Production of human antibodies from transgenic mice / C. Geoffrey Davis
    • Selection of internalizing antibodies for drug delivery / James D. Marks
    • Engineering multivalent antibody fragments for in vivo targeting / Anna M. Wu
    • Production of recombinant bispecific antibodies / Roland E. Kontermann
    • pt. 3. Antibody expression and optimization
    • Expression and isolation of recombinant antibody fragments in E. coli / Keith A. Charlton
    • Expression of recombinant antibodies in mammalian cell lines / Paul J. Yazaki
    • Human antibody production using insect-cell expression systems / Mary C. Guttieri
    • Antibody production in transgenic plants / Eva Stoger
    • Directed mutagenesis of antibody variable domains / Kevin Brady
    • Antibody affinity maturation by chain shuffling / James D. Marks
    • Antibody affinity maturation by random mutagenesis / Ikuo Fujii
    • Developing a minimally immunogenic humanized antibody by SDR grafting / Syed V.S. Kashmiri
    • pt. 4. Antibody characterization and novel applications
    • Antibody purification by column chromatography / Andrea Murray
    • Affinity measurement using surface plasmon resonance / Robert Karlsson
    • Kinetic exclusion assays to study high-affinity binding interactions in homogenous solutions / Robert C. Blake II
    • Characterization of antibody-antigen interactions by fluorescence spectroscopy / Sotiris Missailidis
    • Antibody epitope mapping using arrays of synthetic peptides / Ulrich Reineke
    • Mapping antibody: antigen interactions by mass spectrometry and bioinformatics / Alexandra Huhalov
    • Radiometal labeling of antibodies and antibody fragments for imaging and therapy / Ilse Novak-Hofer
    • Production and characterization of anti-cocaine catalytic antibodies / Paloma de Prada
    • Recombinant immunotoxins in the treatment of cancer / Ira Pastan
    • Antibodies in proteomics / Andrew R.M. Bradbury
    • Targeting of antibodies using aptamers / Sotiris Missailidis.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 1588290921 ((alk. paper))
    • 9781588290922 ((alk. paper))
    • 1592596665 ((eISBN))
    • 9781592596669 ((eISBN))
    LCCN
    2003020281
    OCLC
    53051674
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