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Antibody engineering : methods and protocols / edited by Benny K.C. Lo.
Format
Book
Language
English
Published/Created
Totowa, N.J. : Humana Press, ©2004.
Description
xiv, 561 pages : illustrations ; 24 cm.
Availability
Available Online
Online Content
Online Content
Springer Nature - Complete Protocols
Copies in the Library
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Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.248
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Details
Subject(s)
Immunotechnology
—
Laboratory manuals
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Related name
Lo, Benny K. C.
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Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 248.
[More in this series]
Methods in molecular biology ; 248
Summary note
With 10 recent FDA approvals of therapeutic antibody drugs and hundreds more in the biotechnology pipeline, it is clear that engineered antibodies will play a major role in treating human diseases in the 21st century. In Antibody Engineering: Methods and Protocols, leading researchers and clinicians present a core collection of cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the generation of human recombinant antibodies by humanization approaches, molecular display technologies, and transgenic animals. The techniques for antibody expression and optimization utilize not only bacterial and mammalian cell cultures, but also insect cell cultures and transgenic plants. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics. Each fully tested protocol is described in step-by-step detail and includes background material, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. Comprehensive and up-to-date, Antibody Engineering: Methods and Protocols provides both laboratory and clinical investigators with a diverse array of the fundamental techniques upon which new and imaginative drug technologies can be successfully developed.
Bibliographic references
Includes bibliographical references and index.
Contents
pt. 1. Antibody sequence and structure
Internet resources for the antibody engineer / Benny K.C. Lo
The Kabat database and a bioinformatics example / George Johnson
IMGT, the International ImMunoGeneTics Information Sysmtem, http://imgt.cines.fr / Marie-Paule Lefranc
Antibody variable regions: toward a unified modeling method / Nicholas Whitelegg
Studying antibody conformations by ultracentrifugation and hydrodynamic modeling / Stephen E. Harding
pt. 2. Antibody-lead generation
PCR cloning of human immunoglobulin genes / James D. Marks
Antibody humanization by CDR grafting / Benny K.C. Lo
Selection of human antibodies from phage display libraries / James D. Marks
Production of human single-chain antibodies by ribosome display / Mingyue He
Production of human antibodies from transgenic mice / C. Geoffrey Davis
Selection of internalizing antibodies for drug delivery / James D. Marks
Engineering multivalent antibody fragments for in vivo targeting / Anna M. Wu
Production of recombinant bispecific antibodies / Roland E. Kontermann
pt. 3. Antibody expression and optimization
Expression and isolation of recombinant antibody fragments in E. coli / Keith A. Charlton
Expression of recombinant antibodies in mammalian cell lines / Paul J. Yazaki
Human antibody production using insect-cell expression systems / Mary C. Guttieri
Antibody production in transgenic plants / Eva Stoger
Directed mutagenesis of antibody variable domains / Kevin Brady
Antibody affinity maturation by chain shuffling / James D. Marks
Antibody affinity maturation by random mutagenesis / Ikuo Fujii
Developing a minimally immunogenic humanized antibody by SDR grafting / Syed V.S. Kashmiri
pt. 4. Antibody characterization and novel applications
Antibody purification by column chromatography / Andrea Murray
Affinity measurement using surface plasmon resonance / Robert Karlsson
Kinetic exclusion assays to study high-affinity binding interactions in homogenous solutions / Robert C. Blake II
Characterization of antibody-antigen interactions by fluorescence spectroscopy / Sotiris Missailidis
Antibody epitope mapping using arrays of synthetic peptides / Ulrich Reineke
Mapping antibody: antigen interactions by mass spectrometry and bioinformatics / Alexandra Huhalov
Radiometal labeling of antibodies and antibody fragments for imaging and therapy / Ilse Novak-Hofer
Production and characterization of anti-cocaine catalytic antibodies / Paloma de Prada
Recombinant immunotoxins in the treatment of cancer / Ira Pastan
Antibodies in proteomics / Andrew R.M. Bradbury
Targeting of antibodies using aptamers / Sotiris Missailidis.
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Other format(s)
Also available in an electronic version.
ISBN
1588290921 ((alk. paper))
9781588290922 ((alk. paper))
1592596665 ((eISBN))
9781592596669 ((eISBN))
LCCN
2003020281
OCLC
53051674
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Antibody engineering [electronic resource] : methods and protocols / edited by Benny K.C. Lo.
id
9961543743506421
Antibody Engineering [electronic resource] : Methods and Protocols / edited by Benny K. C. Lo.
id
99125262256206421