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Protein purification protocols.
Format
Book
Language
English
Εdition
2nd ed. / edited by Paul Cutler.
Published/Created
Totowa, N.J. : Humana Press, ©2004.
Description
xii, 484 pages : illustrations ; 26 cm.
Availability
Available Online
Online Content
Online Content
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.244
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Details
Subject(s)
Proteins
—
Purification
—
Laboratory manuals
[Browse]
Related name
Cutler, Paul, 1962-
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Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 244.
[More in this series]
Methods in molecular biology, 1064-3745 ; v. 244
Summary note
In this new edition, the author completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. It brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and thie functions today.
Bibliographic references
Includes bibliographical references and index.
Contents
General strategies / S. Doonan and P. Cutler
Preparation of extracts from animal tissues / J.M. Skehel
Protein extraction from plant tissues / R.J. Fido [and others]
Extraction of recombinant protein from bacteria / A.F. McGettrick and D.M. Worrall
Protein extraction from fungi / P.D. Bridge, T. Kokubun and M.S. Simmonds
Subecllular fractionation of animal tissues / N.M. Ryan
Subcellular fractionation of plant tissues: isolation of plastids and mitochondria / A.K. Tobin and C.G. Bowsher
The extraction of enzymes from plant tissues rich in phenolic compounds / W.S. Pierpoint
Avoidance of proteolysis in extracts / R.J. Beynon and S. Oliver
Concentration of extracts / S. Doonan
Making and changing buffers / S. Doonan
Purification and concentration by ultrafiltration / P. Schratter
Bulk purification by fractional precipitation / S. Doonan
Ion-exchange chromatography / C. Selkirk
Hydrophobic interaction chromatography / P.A. O'Farrell
Affinity chromatography / P. Cutler
Dye-ligand affinity chromatography / A.F. McGettrick and D.M. Worrall
Lectin affinity chromatography / I. West and O. Goldring
Immunoaffinity chromatography / P. Cutler
Immobilized metal-ion affinity chromatography / T.T. Yip and T.W. Hutchens
Chromatography on hydroxyapatite / S. Doonan
Thiophilic affinity chromatography and related methods / P. Matejtschuk
Affinity precipitation methods / J.A. Irwin and K.F. Tipton
Isoelectric focusing / R. Westermeier
Chromatofocusing / T.J. Mantle and P. Noone
Size-exclusion chromatography / P. Cutler
Fast protein liquid chromatography / D. Sheehan and S. O'Sullivan
Reversed-phase chromatography of proteins / W.A. Neville
Extraction of membrane proteins / K. Ohlendieck
Removal of detergent from protein fractions / K. Ohlendieck
Purification of membrane proteins / K. Ohlendieck
Lyophilization of proteins / C. O'Fagain
Storage of pure proteins / C. O'Fagain
Electroelution of proteins from polyacrylamide gels / M.J. Dunn
Electroblotting of proteins from polyacrylamide gels / M.J. Dunn
Two-dimensional polyacrylamide gel electrophoresis for proteome analyses / N.A. Jones
Microscale solution isoelectrofocusing: a sample prefractionation method for comprehensive proteome analysis / X. Zuo and D.W. Speicher
Practical column chromatography / S. Doonan
Detection methods / J. Mozdzanowski and S. Burman
Peptide proteomics / D.E. McNulty and J.R. Slemmon
Multidimensional liquid chromatography of proteins / R. Watson and T. Nadler
Mass spectrometry / D.J. Bell
Purification of therapeutic proteins / J. Bonnerjea
Purification process scale-up / K. Prince and M. Smith.
Show 41 more Contents items
Other format(s)
Also available in an electronic version.
ISBN
1588290670 ((alk. paper))
9781588290670 ((alk. paper))
159259655X ((e-ISBN))
9781592596553 ((e-ISBN))
LCCN
2003006803
OCLC
51936833
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Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Supplementary Information
Other versions
Protein purification protocols [electronic resource].
id
9961541703506421
Protein Purification Protocols [electronic resource] / edited by Paul Cutler.
id
99125171415006421