Transgenic plants : methods and protocols / edited by Leandro Peña.

Format
Book
Language
English
Published/​Created
Totowa, N.J. : Humana Press, ©2005.
Description
xiv, 437 pages : illustrations (some color) ; 24 cm.

Availability

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Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.286 Browse related items Request

    Details

    Subject(s)
    Library of Congress genre(s)
    Series
    • Methods in molecular biology (Clifton, N.J.) ; v. 286. [More in this series]
    • Methods in molecular biology ; v. 286
    Summary note
    A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology??? series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • Transgenic plants: an historical perspective / Luis Herrera-Estrella, June Simpson, and Miguel Martínez-Trujillo
    • Plant transformation: agrobacterium-mediated gene transfer / Abhaya M. Dandekar and Henry J. Fisk
    • Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes-mediated transformation / Mary C. Christey and Robert H. Braun
    • Stable transformation of plant cells by particle bombardment/biolistics /Julie R. Kikkert, José R. Vidal, and Bruce I. Reisch
    • Electroporation: introduction and expression of transgenes in plant protoplasts / Henry J. Fisk and Abhaya M. Dandekar
    • Floral dip: agrobacterium-mediated germ line transformation / Steven J. Clough
    • Production of transgenic crops by the floral-dip method / Ian S. Curtis
    • Chloroplast genetic engineering to improve agronomic traits / Henry Daniell, Oscar N. Ruiz, and Amit Dhingra
    • Organogenesis from transformed tomato explants / Anne Frary and Joyce Van Eck
    • Genetic transformation of conifers utilizing somatic embryogenesis / Krystyna Klimaszewska, Robert G. Rutledge, and Armand Séguin
    • Regeneration of transgenic cassava from transformed embryogenic tissues / Peng Zhang and Johanna Puonti-Kaerlas
    • Genetic transformation of mature citrus plants / Magdalena Cervera [and others]
    • Selectable markers: antibiotic and herbicide resistance / Julia L. Goodwin [and others]
    • Histochemical and fluorometric assays for uidA (GUS) gene detection / Magdalena Cervera
    • Green fluorescent protein quantification in whole plants / Matthew D. Halfhill, Reginald J. Millwood, and C. Neal Stewart, Jr.
    • Positive selection / Allan Wenck and Geneviève Hansen
    • Elimination of marker genes from transgenic plants using MAT vector systems / Hiroyasu Ebinuma [and others]
    • Simple and efficient removal of marker genes from plastids by homologous recombination / Anil Day [and others]
    • The study of transgene copy number and organization / David J. Ingham
    • Analysis of gene expression in transgenc plants / Andrew F. Page and Subhash C. Minocha
    • Transgene integration: use of matrix attachment regions / George C. Allen, Steven Spiker, and William F. Thompson
    • Fluorescence in situ hybridization to localize transgenes in plant chromosomes / Wendy A. Harwood [and others]
    • Amplification of genomic sequences flanking T-DNA insertions by thermal asymmetric interlaced polymerase chain reaction / Yao-Guang Liu, Yuanlin Chen, and Qunyu Zhang
    • Agrobacterium persistence in plant tissues after transformation / Jaime Cubero and María M. López
    • Transgene dispersal through pollen / Laura C. Hudson, Matthew D. Halfhill, and C. Neal Stewart, Jr.
    • Transgenic crops: the current and next generations / Jim M. Dunwell
    • Comparative development and impact of transgenic papayas in Hawaii, Jamaica, and Venezuela / Gustavo Fermín [and others].
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 1588292630 ((alk. paper))
    • 9781588292636 ((alk. paper))
    • 1592598277 ((e-ISBN))
    • 9781592598274 ((e-ISBN))
    LCCN
    2004042471
    OCLC
    54415565
    International Article Number
    • 9781588292636
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