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Transgenic plants : methods and protocols / edited by Leandro Peña.
Format
Book
Language
English
Published/Created
Totowa, N.J. : Humana Press, ©2005.
Description
xiv, 437 pages : illustrations (some color) ; 24 cm.
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Online Content
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Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.286
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Subject(s)
Transgenic plants
—
Laboratory manuals
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Transgenic plants
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Related name
Peña, Leandro
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Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 286.
[More in this series]
Methods in molecular biology ; v. 286
Summary note
A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology??? series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Bibliographic references
Includes bibliographical references and index.
Contents
Transgenic plants: an historical perspective / Luis Herrera-Estrella, June Simpson, and Miguel Martínez-Trujillo
Plant transformation: agrobacterium-mediated gene transfer / Abhaya M. Dandekar and Henry J. Fisk
Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes-mediated transformation / Mary C. Christey and Robert H. Braun
Stable transformation of plant cells by particle bombardment/biolistics /Julie R. Kikkert, José R. Vidal, and Bruce I. Reisch
Electroporation: introduction and expression of transgenes in plant protoplasts / Henry J. Fisk and Abhaya M. Dandekar
Floral dip: agrobacterium-mediated germ line transformation / Steven J. Clough
Production of transgenic crops by the floral-dip method / Ian S. Curtis
Chloroplast genetic engineering to improve agronomic traits / Henry Daniell, Oscar N. Ruiz, and Amit Dhingra
Organogenesis from transformed tomato explants / Anne Frary and Joyce Van Eck
Genetic transformation of conifers utilizing somatic embryogenesis / Krystyna Klimaszewska, Robert G. Rutledge, and Armand Séguin
Regeneration of transgenic cassava from transformed embryogenic tissues / Peng Zhang and Johanna Puonti-Kaerlas
Genetic transformation of mature citrus plants / Magdalena Cervera [and others]
Selectable markers: antibiotic and herbicide resistance / Julia L. Goodwin [and others]
Histochemical and fluorometric assays for uidA (GUS) gene detection / Magdalena Cervera
Green fluorescent protein quantification in whole plants / Matthew D. Halfhill, Reginald J. Millwood, and C. Neal Stewart, Jr.
Positive selection / Allan Wenck and Geneviève Hansen
Elimination of marker genes from transgenic plants using MAT vector systems / Hiroyasu Ebinuma [and others]
Simple and efficient removal of marker genes from plastids by homologous recombination / Anil Day [and others]
The study of transgene copy number and organization / David J. Ingham
Analysis of gene expression in transgenc plants / Andrew F. Page and Subhash C. Minocha
Transgene integration: use of matrix attachment regions / George C. Allen, Steven Spiker, and William F. Thompson
Fluorescence in situ hybridization to localize transgenes in plant chromosomes / Wendy A. Harwood [and others]
Amplification of genomic sequences flanking T-DNA insertions by thermal asymmetric interlaced polymerase chain reaction / Yao-Guang Liu, Yuanlin Chen, and Qunyu Zhang
Agrobacterium persistence in plant tissues after transformation / Jaime Cubero and María M. López
Transgene dispersal through pollen / Laura C. Hudson, Matthew D. Halfhill, and C. Neal Stewart, Jr.
Transgenic crops: the current and next generations / Jim M. Dunwell
Comparative development and impact of transgenic papayas in Hawaii, Jamaica, and Venezuela / Gustavo Fermín [and others].
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Other format(s)
Also available in an electronic version.
ISBN
1588292630 ((alk. paper))
9781588292636 ((alk. paper))
1592598277 ((e-ISBN))
9781592598274 ((e-ISBN))
LCCN
2004042471
OCLC
54415565
International Article Number
9781588292636
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Transgenic Plants [electronic resource] : Methods and Protocols / edited by Leandro Peña.
id
99125198846406421
Transgenic plants [electronic resource] : methods and protocols / edited by Leandro Peña.
id
9961542563506421