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Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual / David C. Amberg, Daniel Burke, Jeffrey Strathern.
Author
Amberg, David C.
[Browse]
Format
Book
Language
English
Εdition
2005 ed.
Published/Created
Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, ©2005.
Description
xvii, 230 pages : illustrations (some color) ; 28 cm
Availability
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Stacks
QK617.5 .K35 2005
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Lewis Library - Stacks
QK617.5 .K35 2005
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Details
Subject(s)
Yeast fungi
—
Genetics
—
Laboratory manuals
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Yeast
—
Genetics
—
Laboratory manuals
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Related name
Cold Spring Harbor Laboratory
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Burke, Dan, 1954-
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Strathern, Jeffrey N.
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Library of Congress genre(s)
Laboratory manuals
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Related work(s)
Burke, Dan, 1954-
Methods in yeast genetics.
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Summary note
"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
Notes
Rev. ed. of: Methods in yeast genetics / Dan Burke, Dean Dawson, Tim Stearns, 2000 ed. 2000.
Bibliographic references
Includes bibliographical references and index.
Contents
Looking at yeast cells
Isolation and characterization of auxotrophic, temperature-sensitive, and osmotic-sensitive mutants
Meiotic mapping
Mitotic recombination and random spore analysis
Transformation of yeast
Synthetic lethal mutants
Gene replacement
Isolation of ras2 suppressors
Manipulating cell types
Isolating mutants by insertional mutagenesis
Isolation of separation of function mutants by two-hybrid differential interaction screening
High-efficiency transformation of yeast
"Quick and dirty" plasmid transformation of yeast colonies
Yeast DNA isolations
Yeast protein extracts
TAP purification
Yeast RNA isolations
Hydroxylamine mutagenesis of plasmid DNA
Assay of beta-galactosidase in yeast
Plate assay for carboxypeptidase Y
Random spore analysis
Yeast vital stains
Yeast immunofluorescence
Actin staining in fixed cells
PCR-mediated gene disruption
Yeast colony PCR
Measuring yeast cell density by spectrophotometry
Cell synchrony
Chromatin immunoprecipitation
Flow cytometry of yeast DNA
Logarithmic growth
EMS mutagenesis
Tetrad dissection
Making a tetrad dissection needle
Picking zygotes
Determining plating efficiency
DNA miniprep from e. coli
Preparing and transforming competent e. coli
Storing and handling the systematic deletion collection
Media
Stock preservation
Yeast genetic and physical maps
Templates for making streak plates
Electrophoretic karyotypes of strains for southern blot mapping
Strains
Counting yeast cells with a standard hemocytometerchamber
Tetrad scoring sheet.
Show 44 more Contents items
ISBN
0879697288 ((pbk. ; : alk. paper))
9780879697280 ((pbk. ; : alk. paper))
LCCN
2004028765
OCLC
57202398
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Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual / David C. Amberg, Daniel Burke, Jeffrey Strathern.
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SCSB-5392240