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Proteomics : a Cold Spring Harbor Laboratory course manual / Andrew J. Link, Joshua LaBaer.
Author
Link, Andrew J.
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Format
Book
Language
English
Published/Created
Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, ©2009.
Description
viii, 228 pages : illustrations (some color) ; 29 cm
Availability
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Stacks
QP551 .L53 2009
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Details
Subject(s)
Proteomics
—
Laboratory manuals
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Related name
Cold Spring Harbor Laboratory
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LaBaer, Joshua
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Library of Congress genre(s)
Laboratory manuals
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Summary note
"Based on a popular course at Cold Spring Harbor Laboratory, this new manual assembles cutting-edge protocols, helpful hints, and lecture notes to teach researchers from a wide variety of disciplines the essential methods of proteomics using state-of-the-art instrumentation. Detailed protocols involving protein microarrays, liquid chromatography, high-throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI-TOF, and MudPIT are provided, along with well-illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. Proteomics: A Cold Spring Harbor Laboratory Course Manual can be used both as the basis for a course and as a detailed bench manual for those performing indispensable proteomic experiments. It is authored by Andrew J. Link, Philip C. Andrews, and Joshua LaBaer, all leaders in their fields, who bring complementary expertise to the manual." --Publisher.
Bibliographic references
Includes bibliographical references and index.
Contents
Preface
Introduction
Experiments : Analysis of whole-cell lysates
Purification of protein complexes
Qualitative and quantitative measurement of peptides
Analysis of protein complexes
Phosphopeptide analysis
Multidimensional protein identification
Quantitative mass spectrometry analysis
Analysis and validation of tandem mass spectra
High-throughput cloning of ORFs
Construction of protein microarrays
Using the nucleic acid programmable protein array
Appendices
Index.
Show 12 more Contents items
ISBN
9780879697877 ((pbk. ; : alk. paper))
0879697873 ((pbk. ; : alk. paper))
9780879697938 ((hardcover ; : alk. paper))
0879697938 ((hardcover ; : alk. paper))
LCCN
2008041374
OCLC
256768378
Statement on language in description
Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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