Proteomics : a Cold Spring Harbor Laboratory course manual / Andrew J. Link, Joshua LaBaer.

Author
Link, Andrew J. [Browse]
Format
Book
Language
English
Published/​Created
Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, ©2009.
Description
viii, 228 pages : illustrations (some color) ; 29 cm

Availability

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Lewis Library - Stacks QP551 .L53 2009 Browse related items Request

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    Summary note
    "Based on a popular course at Cold Spring Harbor Laboratory, this new manual assembles cutting-edge protocols, helpful hints, and lecture notes to teach researchers from a wide variety of disciplines the essential methods of proteomics using state-of-the-art instrumentation. Detailed protocols involving protein microarrays, liquid chromatography, high-throughput cloning of expression constructs, IMAC, mass spectrometry, MALDI-TOF, and MudPIT are provided, along with well-illustrated descriptions of experimental procedures and lists of recommended Web sites and reading material. Proteomics: A Cold Spring Harbor Laboratory Course Manual can be used both as the basis for a course and as a detailed bench manual for those performing indispensable proteomic experiments. It is authored by Andrew J. Link, Philip C. Andrews, and Joshua LaBaer, all leaders in their fields, who bring complementary expertise to the manual." --Publisher.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • Preface
    • Introduction
    • Experiments : Analysis of whole-cell lysates
    • Purification of protein complexes
    • Qualitative and quantitative measurement of peptides
    • Analysis of protein complexes
    • Phosphopeptide analysis
    • Multidimensional protein identification
    • Quantitative mass spectrometry analysis
    • Analysis and validation of tandem mass spectra
    • High-throughput cloning of ORFs
    • Construction of protein microarrays
    • Using the nucleic acid programmable protein array
    • Appendices
    • Index.
    ISBN
    • 9780879697877 ((pbk. ; : alk. paper))
    • 0879697873 ((pbk. ; : alk. paper))
    • 9780879697938 ((hardcover ; : alk. paper))
    • 0879697938 ((hardcover ; : alk. paper))
    LCCN
    2008041374
    OCLC
    256768378
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