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Type 2 diabetes : methods and protocols / edited by Claire Stocker.
Format
Book
Language
English
Published/Created
New York ; London : Humana Press, 2009.
Description
x, 278 p. : ill. (some col.) ; 26 cm.
Availability
Available Online
SpringerNature Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.560
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Details
Subject(s)
Type 2 diabetes
—
Laboratory manuals
[Browse]
Type 2 diabetes
—
Susceptibility
—
Research
[Browse]
Type 2 diabetes
—
Research
[Browse]
Type 2 diabetes
[Browse]
Pancreas
[Browse]
Insulin
[Browse]
Mice
[Browse]
Related name
Stocker, Claire
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 560.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 560
Springer protocols
Summary note
Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.
Bibliographic references
Includes bibliographical references and index.
Contents
1. Selecting the "right" mouse model for metabolic syndrome and type 2 diabetes research / Edward H. Leiter
2. Nutritional models of type 2 diabetes mellitus / Beverly Sara Muhlhausler
3. The isolation and purification of rodent pancreatic islets of Langerhans / Jacqueline F.O ̓Dowd
4. The measurement of insulin secretion from isolated rodent islets of Langerhans / Anna L. Nolan and Jacqueline F.O ̓Dowd
5. The incubation and monitoring of cell viability in primary rat islets of Langerhans and pancreatic B-cell lines / Noel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell
6. In vitro culture of isolated islets of Langerhans : analysis of function / Anna L. Nolan
7. Single cell RT-PRC identification of genes expressed by human islet endocrine cells / Dany Muller, Peter M. Jones, and Shanta J. Persaud
8. Laser capture microdissection of human pancreatic B-cells and RNA preparation for gene expression profiling / Lorella Marselli [and others]
9. In vitro transdifferentiation of human hepatoma cells into pancreatic-like cells / Wan-chun Li
10. The measurement of GLUT4 translocation in 3T3-L1 adipocytes / Nicky Konstantopoulos and Juan Carlos Molero-Navajas
11. Pancreatic remodeling : beta cell apoptosis, proliferation and neogenesis and the measurement of beta cell mass and of individual beta cell size / Eduard Montanya and Noélia Téllez
12. Morphology of pancreatic islets : a time course of pre-diabetes in Zucker fatty rats / Petra Augstein and Eckhard Salzsieder
13. Fluorescent Immunohistochemistry and in situ hybridization analysis of pancreas / Xiuli Wang, Shundi Ge, and Gay M. Crooks
14. The measurement of insulin secretion using pancreas perfusion in the rodent / Edward T. Wargent
15. Hyperinsulinemic-euglycemic clamp to assess insulin sensitivity in vivo / Jason K. Kim
16. Gene expression analysis in diabetes research / Peter White and Klaus H. Kaestner
17. Gene expression mining in type 2 diabetes research / Donald R. Dunbar.
Show 14 more Contents items
ISBN
9781934115152 ((hbk.))
1934115150 ((hbk.))
9781597454483 ((ebk.))
1597454486 ((ebk.))
LCCN
2009922609
OCLC
319204986
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Type 2 Diabetes [electronic resource] : Methods and Protocols / edited by Claire Stocker.
id
99125287327806421
Type 2 diabetes : methods and protocols / edited by Claire Stocker.
id
SCSB-5491088