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Heterologous gene expression in E. coli : methods and protocols / edited by Thomas C. Evans, Jr., Ming-Qun Xu.
Format
Book
Language
English
Published/Created
New York, NY : Humana Press, ©2011.
Description
xi, 310 pages : illustrations ; 27 cm
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Copies in the Library
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Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.705
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Details
Subject(s)
Gene expression
—
Laboratory manuals
[Browse]
Escherichia coli
—
Genetics
—
Laboratory manuals
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Gene expression
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Genetics
—
Technique
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Related name
Evans, Thomas C.
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Xu, Ming-Qun
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Library of Congress genre(s)
Laboratory manuals
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Series
Springer protocols (Series)
[More in this series]
Methods in molecular biology (Clifton, N.J.) ; v. 705.
[More in this series]
Springer protocols
Methods in molecular biology, 1064-3745 ; 705
Summary note
Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology"!format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host
Bibliographic references
Includes bibliographical references and index.
Contents
Adjustment of codon usage frequencies by codon harmonization improves protein expression and folding / Evelina Angov, Patricia M. Legler, and Ryan M. Mease
SUMO fusion technology for enhanced protein expression and purification in prokaryotes and eukaryotes / Raymond J. Peroutka III, Steven J. Orcutt, James E. Strickler, and Tauseef R. Butt
Molecular and chemical chaperones for improving the yields of soluble recombinant proteins / Ario de Marco
Genetic selection of solubility-enhanced proteins using the twin-arginine translocation system / Adam C. Fisher, Mark A. Rocco, and Matthew P. DeLisa
Protein folding liquid chromatography / Quan Bai and Xindu Geng
Site-specific protein labeling by intein-mediated protein ligation / Inca Ghosh, Nancy Considine, Elissa Maunus, Luo Sun, Aihua Zhang, John Buswell, Thomas C. Evans, Jr., and Ming-Qun Xu
Efficient expression of human aromatase (CYP19) in E. coli / Norio Kagawa
Expression of recombinant cytochromes c in E. coli / Yuri Y. Londer
Semi-synthesis of glycoprotiens from E. coli through native chemical ligation / Jonathan P. Richardson and Derek Macmillan
Expression of recombinant proteins with uniform N-termini / Orsolya Király, Lan Guan, and Miklós Sahin-Tóth
Recent developments in difficult protein expression : a guide to E. coli strains, promoters, and relevant host mutations / James C. Samuelson
Periplasmic chaperones used to enhance functional secretion of proteins in E. coli / Martin Schlapschy and Arne Skerra
Engineering unusual amino acids into peptides using lantibiotic synthetase / Jun-ichi Nagao, Kouki Shioya, Yoshitaka Harada, Ken-ichi Okuda, Takeshi Zendo, Jiro Nakayama, and Kenji Sonomoto
The targeted expression of nucleotide sugar transporters to the E. coli inner membrane / Joe Tiralongo and Andrea Maggioni
Detection of protein-protein interactions in bacteria by GFP-fragment reconstitution / Akira Kanno, Takeaki Ozawa, and Yoshio Umezawa
Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner / Ping Sun, Joseph E. Tropea, and David S. Waugh
Introducing predetermined mutations throughout a target gene using TDEM (Transposon-Directed Base-Exchange Mutagenesis) / Yun Cheol Kim
Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferase / Aihua Zhang, Luo Sun, John Buswell, Nancy Considine, Inca Ghosh, Anastasiya Masharina, Christopher Noren, and Ming-Qun Xu.
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Other format(s)
Also available in an electronic version.
ISBN
9781617379666 ((alk. paper))
1617379662 ((alk. paper))
1617379670
9781617379673
LCCN
2011283567
OCLC
694879463
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Heterologous Gene Expression in E.coli [electronic resource] : Methods and Protocols / edited by Thomas C. Evans, Jr., Ming-Qun Xu.
id
99125221949006421
Heterologous gene expression in E. coli : methods and protocols / edited by Thomas C. Evans, Jr., Ming-qun Xu.
id
SCSB-8855584