Cell-based microarrays : methods and protocols / edited by Ella Palmer.

Format
Book
Language
English
Published/​Created
New York : Humana Press, ©2011.
Description
x, 209 pages : illustrations (some color) ; 27 cm

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Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.706 Browse related items Request

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    Summary note
    (Publisher-supplied data) As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • Cell-based microarrays : overview / Ella Palmer
    • Cell-based co-transfection microarrays for use with HEK293T cells on a poly D-lysine-coated polystyrene microplate / Meenal Soni and Fang Lai
    • Large-scale cell-based microarrays and their use with HEK293T cells and downstream apoptotic assays / Ella Palmer and Tom C. Freeman
    • A novel fluorescent transcriptional reporter for cell-based microarray assays / Tanya M. Redmond and Michael D. Uhler
    • High-throughput subcellular protein localization using transfected-cell arrays / Yuhui Hu and Michal Janits
    • Cell arrays for the measurement of organelle dynamics in living cells / Holger Erfle [and others]
    • High-throughput immunofluorescence microscopy using yeast spheroplast cell-based microarrays / Wei Niu, G. Traver Hart, and Edward M. Marcotte
    • Cell-based microarrays of infectious adenovirus encoding short hairpin RNA (shRNA) / Hansjürgen Volkmer and Frank Weise
    • Reverse transfected cell microarrays in infectious disease research / Andreas Konrad [and others]
    • Transfected cell microarrays for the expression of membrane-displayed single-chain antibodies / Baochuan Lin and James B. Delehanty --. Blood cell capture on antibody microarrays and monitoring of the cell caputure using surface plasmon resonance imaging / Yoann Roupioz [and others]
    • Immobilized culture and transfection microarry of non-adherent cells / Satoshi Yamaguchi, Erika Matsunuma, and Teruyuki Nagamune
    • Plasma polymer and PEG-based coatings for DNA, protein and cell microarrays / Andrew L. Hook, Nicolas H. Voelcker, and Helmut Thissen
    • Polymer microarrays for cellular high-content screening / Salvatore Pernagallo and Juan J. Dias-Mochon
    • High-throughput analyses of gene functions on a cell chip by electroporation / Koichi Kato and Hiroo Iwata
    • Microfluidic image cytometry / Ken-ichiro Kamei [and others].
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781617379697 ((alk. paper))
    • 1617379697 ((alk. paper))
    • 9781617379703 ((e-ISBN))
    • 1617379700 ((e-ISBN))
    LCCN
    2011282746
    OCLC
    668191295
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