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Mammalian chromosome engineering : methods and protocols / edited by Gyula Hadlaczky.
Format
Book
Language
English
Published/Created
New York : Humana, ©2011.
Description
ix, 257 pages : illustrations ; 27 cm
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Online Content
Copies in the Library
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Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506.M45 1984 vol.738
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Details
Subject(s)
Genetic engineering
—
Laboratory manuals
[Browse]
Mammals
—
Genetics
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Genetics
—
Technique
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Related name
Hadlaczky, Gyula
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Library of Congress genre(s)
Laboratory manuals
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Series
Springer protocols (Series)
[More in this series]
Methods in molecular biology (Clifton, N.J.) ; v. 738.
[More in this series]
Springer protocols
Methods in molecular biology, 1064-3745 ; v. 738
Summary note
[Publisher-supplied data] The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry. Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system. Written in the highly successful Methods in Molecular Biology series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.
Bibliographic references
Includes bibliographical references and index.
Contents
Developing Extrachromosomal Gene Expression Vector Technologies: An Overview
High Capacity Extrachromosomal Gene Expression Vectors
Naturally Occurring Minichromosome Platforms in Chromosome Engineering: An Overview
Chromosome Transfer Via Cell Fusion
Insertional Engineering of Chromosomes with Sleeping Beauty Transposition: An Overview
Rodent Transgenesis Mediated by a Novel Hyperactive Sleeping Beauty Transposon System
Construction and Use of a Bottom-Up HAC Vector for Transgene Expression
De novo Generation of Satellite DNA-Based Artificial Chromosomes by Induced Large-Scale Amplification
Downstream Bioengineering of ACE Chromosomes for Incorporation of Site-Specific Recombination Cassettes
Chromosome Engineering with Lambda-Integrase Mediated Recombination System: The ACE System
Dendrimer Mediated Transfer of Engineered Chromosomes
Engineered Chromosomes in Transgenics
Transfer of Stem Cells Carrying Engineered Chromosomes with XY Clone Laser System
Mammalian Artificial Chromosomes and Clinical Applications for Genetic Modification of Stem Cells: An Overview
Engineered Mammalian Chromosomes in Cellular Protein Production: Future Prospects.
Show 12 more Contents items
Other format(s)
Also available in an electronic version.
ISBN
9781617790980 ((alk. paper))
1617790982 ((alk. paper))
9781617790997 ((e-ISBN))
1617790990 ((e-ISBN))
LCCN
2011923965
OCLC
706626327
Statement on language in description
Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Other versions
Mammalian Chromosome Engineering [electronic resource] : Methods and Protocols / edited by Gyula Hadlaczky.
id
99125134712406421
Mammalian chromosome engineering [electronic resource] : methods and protocols / edited by Gyula Hadlaczky.
id
9965618163506421