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G protein-coupled receptor signaling in plants : methods and protocols / edited by Mark P. Running.
Format
Book
Language
English
Published/Created
New York : Humana Press, [2013]
Description
x, 162 pages : illustrations (some color) ; 26 cm.
Availability
Available Online
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1043
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Details
Subject(s)
Plant cellular signal transduction
—
Laboratory manuals
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G proteins
—
Laboratory manuals
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Cellular signal transduction
[Browse]
Editor
Running, Mark P.
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Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 1043.
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Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 1043
Springer protocols
Summary note
Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins. Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.
Bibliographic references
Includes bibliographical references and index.
Contents
Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs
Measurement of GTP-Binding and GTPase Activity of Heterotrimeric G[alpha] Proteins
Biochemical Analysis of the Interaction Between Phospholipase D[alpha]1 and GTP-Binding Protein [alpha]-Subunit from Arabidopsis thaliana
Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants
Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis
Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis
Fusarium oxysporum Infection Assays in Arabidopsis
Analysis of Unfolded Protein Response in Arabidopsis
Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis
G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings
Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana
In Vivo Localization in Arabidopsis Protoplasts and Root Tissue
Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography-Coupled Mass Spectrometry
In Vitro Myristoylation Assay of Arabidopsis Proteins
Assaying Protein S-Acylation in Plants
In Vitro Prenylation Assay of Arabidopsis Proteins.
Show 13 more Contents items
ISBN
9781627035316 ((alk. paper))
1627035311 ((alk. paper))
LCCN
2013942971
OCLC
839397563
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G Protein-Coupled Receptor Signaling in Plants [electronic resource] : Methods and Protocols / edited by Mark P. Running.
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