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Legume genomics : methods and protocols / edited by Ray J. Rose.
Format
Book
Language
English
Published/Created
New York : Humana Press, [2013]
©2013
Description
xi, 316 pages : illustrations (some color) ; 27 cm.
Availability
Available Online
SpringerNature Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1069
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Details
Subject(s)
Legumes
—
Genetics
—
Laboratory manuals
[Browse]
Editor
Rose, Ray J.
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1069.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 1069
Springer protocols, 1949-2448
Summary note
Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.
Bibliographic references
Includes bibliographical references and index.
Contents
The Model Legume Genomes
Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization
Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes
RNA-Seq for Transcriptome Analysis in Non-Model Plants
Functional Analysis of Legume Genome Arrays
Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing
Determining Abundance of MicroRNAs and Other Small RNAs in Legumes
Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines
Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection
High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants
Isolation and Forward Genetic Analysis of Developmental Genes in Pea
Gene Silencing in Medicago truncatula Roots Using RNAi
Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius)
Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens
Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration
Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins
Proteomics and the Analysis of Nodulation
Phosphoproteomic Analysis of Peptides
Plant Metabolomics: From Experimental Design to Knowledge Extraction.
Show 16 more Contents items
Other format(s)
Also available in an electronic version.
ISBN
9781627036122 ((acid-free paper))
1627036121 ((acid-free paper))
9781627036139 ((eBook))
162703613X ((eBook))
LCCN
2013946542
OCLC
843859292
Statement on language in description
Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Legume Genomics [electronic resource] : Methods and Protocols / edited by Ray J. Rose.
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99125243571106421