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DNA electrophoresis : methods and protocols / edited by Svetlana Makovets.
Format
Book
Language
English
Published/Created
New York : Humana Press, [2013]
Description
x, 299 pages : illustrations (some color) 28 cm.
Availability
Available Online
SpringerNature Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1054
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Details
Subject(s)
Electrophoresis
—
Laboratory manuals
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DNA
—
Laboratory manuals
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Editor
Makovets, Svetlana
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Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 1054.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in Molecular Biology, 1064-3745 ; 1054
Springer protocols
Summary note
In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.
Bibliographic references
Includes bibliographical references and index.
Contents
DNA electrophoresis: historical and theoretical perspectives / Gareth A. Roberts and David T.F. Dryden
Basic DNA electrophoresis in molecular cloning: a comprehensive guide for beginners / Svetlana Makovets
Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis / Nicholas P. Robinson
Analysis of DNA structures from eukaryotic cells by two-dimensional native-native DNA agarose gel electrophoresis / Andreas S. Ivessa
Directionality of replication fork movement determined by two-dimensional native-native DNA agarose gel electrophoresis / Andreas S. Ivessa
Native/denaturing two-dimensional DNA electrophoresis and its application to the analysis of recombination intermediates / Jessica P. Lao, Shangming Tang, and Neil Hunter
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis / Jorge B. Schvartzman [and three others]
A neutral glyoxal gel electrophoresis method for the detection and semi-quantitation of DNA single-strand breaks / Brian Pachkowski and Jun Nakamura
Denaturing gradient gel electrophoresis (DGGE) / Fiona Strathdee and Andrew Free
Polyacrylamide temperature gradient gel electrophoresis / Viktor Viglasky
Separation of DNA oligonucleotides using denaturing urea PAGE / Fiona Flett and Heidrun Interthal
Pulsed-field gel electrophoresis of bacterial chromosomes / Julia S.P. Mawer and David R.F. Leach
Resolution of budding yeast chromosomes using pulsed-field gel electrophoresis / Aziz El Hage and Jonathan Houseley
Analysis of DNA damage via single-cell electrophoresis / Diana Anderson and Julian Laubenthal
Fluorescence in situ hybridization on electrophoresed cells to detect sequence specific DNA damage / Julian Laubenthal and Diana Anderson
Analysis of DNA-protein interactions using PAGE: band-shift assays / Lynn Powell
Assaying cooperativity of protein - DNA interactions using agarose gel electrophoresis / Tanya L. Williams and Daniel L. Levy
DNA bending by proteins: utilizing plasmid pBendAT as a tool / Fenfei Leng
Using PCR coupled to PAGE for detection and semiquantitative evaluation of telomerase activity / Laura Gardano.
Show 16 more Contents items
ISBN
1627035648 ((alk. paper))
9781627035644 ((alk. paper))
LCCN
2013945081
OCLC
841520711
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DNA Electrophoresis [electronic resource] : Methods and Protocols / edited by Svetlana Makovets.
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99125243575206421