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Protein affinity tags : methods and protocols / edited by Richard J. Giannone, Andrew B. Dykstra.
Format
Book
Language
English
Published/Created
New York : Humana Press, [2014]
Description
x, 213 pages : illustrations (some color) ; 26 cm.
Availability
Available Online
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1177
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Details
Subject(s)
Proteins
—
Affinity labeling
—
Laboratory manuals
[Browse]
Proteins
—
Purification
—
Laboratory manuals
[Browse]
Editor
Giannone, Richard J.
[Browse]
Dykstra, Andrew B.
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1177.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular medicine, 1064-3745 ; 1177
Springer protocols, 1949-2448
Summary note
"Protein Affinity Tags: Methods and Protocols provides researchers with the necessary information, tools, and strategy required for proper inquiry into a given protein{́OCLCbr#80}{OCLCbr#99}s function and structure. Today{́OCLCbr#80}{OCLCbr#99}s researchers can easily alter the amino acid sequence of any given protein, a powerful technology allowing for the precise engineering of specific proteins. Protein affinity tagging employs the use of known protein binding interactions in order to {́OCLCbr#80}{OCLCbr#9C}fish out{́OCLCbr#80}? a protein-of-interest or, with more advanced tags, a protein complex. This technology paired with recent advancements in DNA sequencing technology promises a future that is awash with novel genomic information {́OCLCbr#80}? information that will not only expand our knowledge of the processes that govern life, but also fuel innovation that will undoubtedly conquer many of today{́OCLCbr#80}{OCLCbr#99}s most salient health and environmental challenges. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Protein Affinity Tags: Methods and Protocols will be an invaluable resource to those seeking to employ protein affinity tags to study their biological system of interest."--Page [4] of cover.
Bibliographic references
Includes bibliographical references and index.
Contents
1D4 : a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins / Laurie L. Molday and Robert S. Molday
Affinity purification of heme-tagged proteins / Wesley B. Asher and Kara L. Bren
Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag / Dongmei Wang and Jiong Hong
Purification of E. coli proteins using a self-cleaving chitin-binding affinity tag / Michael J. Coolbaugh and David W. Wood
Simplified protein purification using an autoprocessing, inducible enzyme tag / Aimee Shen
SUMO as a solubility tag and in vivo cleavage of SUMO fusion proteins with UlP1 / Dennis Kuo, Minghua Nie, and Albert J Courey
Rescuing aggregation-prone proteins in Escherichia coli with a dual His6-MBP tag / Danielle Needle and David S. Waugh
Expression, purification, and immobilization of recombinant tamavidin 2 fusion proteins Yashimitsu Takakura, Naomi Oka, and Masako Tsunashima
Use of tandem affinity chromatography for purification of cannabinoid receptor CB2 / Silvia C. Locatelli-Hoops and Alexei A. Yeliseev
Detection of protein-protein interactions using tandem affinity purification / Ian Goodfellow and Dalan Bailey
An improved in vivo biotinylation strategy combined with FLAG and antibody based approaches for affinity purification of protein complexes in mouse embryonic stem cells / Francesco Faiolo [and three others]
Purification of recombinant proteins with a multifunctional GFP tag / Takashi Murayama and Takuya Kobayashi
Targeted purification of SnAvi-tagged proteins / Ursula Schäffer, Ralf Baumeister, and Ekkehard Schulze
An efficient fluorescent protein-based multifunctional affinity purification approach in mammalian cells / Hanhui Ma [and three others]
Bimolecular affinity purification : a variation of TAP with multiple applications / Petro Starokadomskyy and Ezra Burstein.
Show 12 more Contents items
ISBN
9781493910335 (alk. paper)
1493910337 (alk. paper)
LCCN
2014939955
OCLC
875742014
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Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Protein Affinity Tags [electronic resource] : Methods and Protocols / edited by Richard J. Giannone, Andrew B. Dykstra.
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99125340866306421