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RNA nanotechnology and therapeutics : methods and protocols / edited by Peixuan Guo, Farzin Haque.
Format
Book
Language
English
Published/Created
New York, NY : Humana Press ; Springer, [2015]
©2015
Description
1 online resource (xi, 239 pages) : illustrations (some color)
Availability
Available Online
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1297
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Details
Subject(s)
RNA
—
Biotechnology
—
Laboratory manuals
[Browse]
Nanomedicine
—
Laboratory manuals
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Medical genetics
—
Laboratory manuals
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Editor
Guo, Peixuan
[Browse]
Haque, Farzin
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v.1297.
[More in this series]
Methods in Molecular Biology, 1064-3745 ; 1297
Summary note
This volume contains a compilation of techniques and laboratory protocols directly related to RNA nanotechnology and its applications in nanobiotechnology and nanomedicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nanoarchitectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nanoconstructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.
Bibliographic references
Includes bibliographical references and index.
Source of description
Online resource; title from PDF title page (SpringerLink, viewed April 29, 2015).
Contents
Overview of methods in RNA nanotechnology : synthesis, purification, and characterization of RNA nanoparticles / Farzin Haque and Peixuan Guo
Multiple approaches for the investigation of bacterial small regulatory RNAs self-assembly / Christophe Lavelle, Florent Busi, and Véronique Arluison
Measuring the elasticity of ribonucleotide(s)-containing DNA molecules using AFM / Kyung Duk Koh ... [et al.]
Silver nanoclusters for RNA nanotechnology : steps towards visualization and tracking of RNA nanoparticle assemblies / Kirill A. Afonin ... [et al.]
Large scale purification of RNA nanoparticles by preparative ultracentrifugation / Daniel L. Jasinski ... [et al.]
HPLC purification of RNA aptamers up to 59 nucleotides with single-nucleotide resolution / Zhen Huang ... [et al.]
Using RNA nanoparticles with thermostable motifs and fluorogenic modules for real-time detection of RNA folding and turnover in prokaryotic and eukaryotic cells / Hui Zhang ... [et al.]
Fluorescence labeling of short RNA by oxidation at the 3'-end / Chen Qiu, Wang-Yi Liu, and Yong-Zhen Xu
Methods and assays for specific targeting and delivery of RNA nanoparticles to cancer metastases / Piotr Rychahou ... [et al.]
Functional assays for specific targeting and delivery of RNA nanoparticles to brain tumor / Tae Jin Lee ... [et al.]
Aptamer-mediated nanoparticle interactions : from oligonucleotide-protein complexes to SELEX screens / Laetitia Evadé ... [et al.]
Methods for assembling B-cell lymphoma specific and internalizing aptamer-siRNA nanoparticles via the sticky bridge / Jiehua Zhou, John J. Rossi, and Ka To Shum
High-throughput screening assay for the functional delivery of splice-switching oligonucleotides in human melanoma cells / John M. Deanand Robert K. DeLong
Design, assembly, and evaluation of RNA-protein nanostructures / Hirohisa Ohno, Eriko Osada, and Hirohide Saito
Mapping RNA interactions to proteins in virions using CLIP-Seq / Baochang Fan, Peng Ni, and C. Cheng Kao
Mapping protein-RNA interactions by RCAP, RNA-cross-linking and peptide fingerprinting / Robert C. Vaughan and C. Cheng Kao.
Show 13 more Contents items
ISBN
9781493925629 (electronic bk.)
1493925628 (electronic bk.)
149392561X
9781493925612
LCCN
2015935947
OCLC
908165078
Doi
10.1007/978-1-4939-2562-9
Statement on language in description
Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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RNA Nanotechnology and Therapeutics [electronic resource] : Methods and Protocols / edited by Peixuan Guo, Farzin Haque.
id
99125287521306421