RNA nanotechnology and therapeutics : methods and protocols / edited by Peixuan Guo, Farzin Haque.

Format
Book
Language
English
Published/​Created
  • New York, NY : Humana Press ; Springer, [2015]
  • ©2015
Description
1 online resource (xi, 239 pages) : illustrations (some color)

Availability

Copies in the Library

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Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.1297 Browse related items Request

    Details

    Subject(s)
    Editor
    Library of Congress genre(s)
    Series
    • Methods in molecular biology (Clifton, N.J.) ; v.1297. [More in this series]
    • Methods in Molecular Biology, 1064-3745 ; 1297
    Summary note
    This volume contains a compilation of techniques and laboratory protocols directly related to RNA nanotechnology and its applications in nanobiotechnology and nanomedicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nanoarchitectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nanoconstructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls. Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.
    Bibliographic references
    Includes bibliographical references and index.
    Source of description
    Online resource; title from PDF title page (SpringerLink, viewed April 29, 2015).
    Contents
    • Overview of methods in RNA nanotechnology : synthesis, purification, and characterization of RNA nanoparticles / Farzin Haque and Peixuan Guo
    • Multiple approaches for the investigation of bacterial small regulatory RNAs self-assembly / Christophe Lavelle, Florent Busi, and Véronique Arluison
    • Measuring the elasticity of ribonucleotide(s)-containing DNA molecules using AFM / Kyung Duk Koh ... [et al.]
    • Silver nanoclusters for RNA nanotechnology : steps towards visualization and tracking of RNA nanoparticle assemblies / Kirill A. Afonin ... [et al.]
    • Large scale purification of RNA nanoparticles by preparative ultracentrifugation / Daniel L. Jasinski ... [et al.]
    • HPLC purification of RNA aptamers up to 59 nucleotides with single-nucleotide resolution / Zhen Huang ... [et al.]
    • Using RNA nanoparticles with thermostable motifs and fluorogenic modules for real-time detection of RNA folding and turnover in prokaryotic and eukaryotic cells / Hui Zhang ... [et al.]
    • Fluorescence labeling of short RNA by oxidation at the 3'-end / Chen Qiu, Wang-Yi Liu, and Yong-Zhen Xu
    • Methods and assays for specific targeting and delivery of RNA nanoparticles to cancer metastases / Piotr Rychahou ... [et al.]
    • Functional assays for specific targeting and delivery of RNA nanoparticles to brain tumor / Tae Jin Lee ... [et al.]
    • Aptamer-mediated nanoparticle interactions : from oligonucleotide-protein complexes to SELEX screens / Laetitia Evadé ... [et al.]
    • Methods for assembling B-cell lymphoma specific and internalizing aptamer-siRNA nanoparticles via the sticky bridge / Jiehua Zhou, John J. Rossi, and Ka To Shum
    • High-throughput screening assay for the functional delivery of splice-switching oligonucleotides in human melanoma cells / John M. Deanand Robert K. DeLong
    • Design, assembly, and evaluation of RNA-protein nanostructures / Hirohisa Ohno, Eriko Osada, and Hirohide Saito
    • Mapping RNA interactions to proteins in virions using CLIP-Seq / Baochang Fan, Peng Ni, and C. Cheng Kao
    • Mapping protein-RNA interactions by RCAP, RNA-cross-linking and peptide fingerprinting / Robert C. Vaughan and C. Cheng Kao.
    ISBN
    • 9781493925629 (electronic bk.)
    • 1493925628 (electronic bk.)
    • 149392561X
    • 9781493925612
    LCCN
    2015935947
    OCLC
    908165078
    Doi
    • 10.1007/978-1-4939-2562-9
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