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T-cell development : methods and protocols / edited by Rémy Bosselut, Melanie S. Vacchio.
Format
Book
Language
English
Published/Created
New York, NY : Humana Press, [2016]
©2016.
Description
1 online resource (xi, 297 pages) : illustrations (some color)
Availability
Available Online
SpringerNature Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1323
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Details
Subject(s)
T cells
—
Laboratory manuals
[Browse]
Editor
Bosselut, Rémy
[Browse]
Vacchio, Melanie S.
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1323.
[More in this series]
Methods in molecular biology, 1940-6029 ; volume 1323
Summary note
This volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Source of description
Online resource; title details screen (SpringerLink, viewed August 28, 2015).
Contents
200 million thymocytes and I: A beginner's survival guide to t cell development
Development of [gamma][delta] T cells, the special-force soldiers of the immune system
Genetic tools to study T cell development
Assessment of T cell development by flow cytometry
Flow cytometry analysis of thymic epithelial cells and their subpopulations
Identifying the spatial relationships of thymic stromal and thymocyte subsets by immunofluorescence analysis
Purification of thymocyte and T cell subsets
Retroviral transduction of T cells and T cell precursors
Bone marrow and fetal liver radiation chimeras
In vitro analyses of T cell effector differentiation
Studying T cell development in thymic slices
FTOC-based analysis of negative selection
Reconstituted thymus organ culture
Induction of T cell development in vitro by delta-like (Dll)-expressing stromal cells
In vitro analysis of thymocyte signaling
Molecular analysis of mouse T cell receptor [alpha] and [beta] gene rearrangements
Intrathymic injection
Analysis of cell proliferation and homeostasis using EdU labeling
Characterization and isolation of human T cell progenitors
Approaches to study human T cell development
Humanized mice to study human T cell development
Using the zebrafish model to study T cell development.
Show 19 more Contents items
ISBN
9781493928095 (electronic bk.)
1493928090 (electronic bk.)
1493928082
9781493928088
OCLC
919312606
Doi
10.1007/978-1-4939-2809-5
Statement on language in description
Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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T-Cell Development [electronic resource] : Methods and Protocols / edited by Rémy Bosselut, Melanie S. Vacchio.
id
99125314441506421