T-cell development : methods and protocols / edited by Rémy Bosselut, Melanie S. Vacchio.

Format
Book
Language
English
Published/​Created
  • New York, NY : Humana Press, [2016]
  • ©2016.
Description
1 online resource (xi, 297 pages) : illustrations (some color)

Availability

Copies in the Library

Location Call Number Status Location Service Notes
Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.1323 Browse related items Request

    Details

    Subject(s)
    Editor
    Library of Congress genre(s)
    Series
    • Methods in molecular biology (Clifton, N.J.) ; v. 1323. [More in this series]
    • Methods in molecular biology, 1940-6029 ; volume 1323
    Summary note
    This volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.
    Source of description
    Online resource; title details screen (SpringerLink, viewed August 28, 2015).
    Contents
    • 200 million thymocytes and I: A beginner's survival guide to t cell development
    • Development of [gamma][delta] T cells, the special-force soldiers of the immune system
    • Genetic tools to study T cell development
    • Assessment of T cell development by flow cytometry
    • Flow cytometry analysis of thymic epithelial cells and their subpopulations
    • Identifying the spatial relationships of thymic stromal and thymocyte subsets by immunofluorescence analysis
    • Purification of thymocyte and T cell subsets
    • Retroviral transduction of T cells and T cell precursors
    • Bone marrow and fetal liver radiation chimeras
    • In vitro analyses of T cell effector differentiation
    • Studying T cell development in thymic slices
    • FTOC-based analysis of negative selection
    • Reconstituted thymus organ culture
    • Induction of T cell development in vitro by delta-like (Dll)-expressing stromal cells
    • In vitro analysis of thymocyte signaling
    • Molecular analysis of mouse T cell receptor [alpha] and [beta] gene rearrangements
    • Intrathymic injection
    • Analysis of cell proliferation and homeostasis using EdU labeling
    • Characterization and isolation of human T cell progenitors
    • Approaches to study human T cell development
    • Humanized mice to study human T cell development
    • Using the zebrafish model to study T cell development.
    ISBN
    • 9781493928095 (electronic bk.)
    • 1493928090 (electronic bk.)
    • 1493928082
    • 9781493928088
    OCLC
    919312606
    Doi
    • 10.1007/978-1-4939-2809-5
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