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Drosophila oogenesis : methods and protocols / edited by Diana P. Bratu, Gerard P. McNeil.
Format
Book
Language
English
Published/Created
New York : Humana Press : Springer, [2015]
©2015
Description
xi, 245 pages : illustrations (some color) ; 27 cm
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Copies in the Library
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Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1328
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Details
Subject(s)
Drosophila
—
Genetics
—
Laboratory manuals
[Browse]
Oogenesis
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Editor
Bratu, Diana P.
[Browse]
McNeil, Gerard P.
[Browse]
Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 1328.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; v. 1328
Springer protocols
Summary note
This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Bibliographic references
Includes bibliographical references and index.
Contents
Drosophila melanogaster oogenesis : an overview / John M. McLaughlin and Diana P. Bratu
Basic techniques in Drosophila ovary preparation / Letitia Thompson, Kristen Randolph, and Amanda Norvell
Mosaic analysis in the Drosophila melanogaster ovary / Thomas Rubin and Jean-René Huynh
Genetic mosaic analysis of stem cell lineages in the Drosophila ovary / Kaitlin M. Laws and Daniela Drummond-Barbosa
Culturing Drosophila egg chambers and investigating developmental processes through live imaging / Lathiena Manning and Michelle Starz-Gaiano
Border cell migration : a model system for live imaging and genetic analysis of collective cell movement / Mohit Prasad [and four others]
Visualizing microtubule networks during Drosophila oogenesis using fixed and live Imaging / Kevin Legent, Nicolas Tissot, and Antoine Guichet
Visualization of actin cytoskeletal dynamics in fixed and live Drosophila egg chambers / Christopher M. Groen and Tina L. Tootle
Single-molecule RNA in situ hybridization (smFISH) and immunofluorescence (IF) in the Drosophila egg chamber / Livia V. Bayer [and three others]
Fluorescent in situ hybridization of nuclear bodies in Drosophila melanogaster ovaries / Zehra F. Nizami, Ji-Long Liu, and Joseph G. Gall
Ultrastructural analysis of Drosophila ovaries by electron microscopy / Thomas R. Hurd [and seven others]
Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries / Shinsuke Shibata [and six others]
Visualizing cytoophidia expression in Drosophila follicle cells via immunohistochemistry / Ömür Y. Tastan and Ji-Long Liu
Detection of cell death and phagocytosis in the Drosophila ovary / Tracy L. Meehan [and three others]
Analysis of cell cycle switches in Drosophila oogenesis / Dongyu Jia , Yi-Chun Huang , and Wu-Min Deng
Global run-on sequencing (GRO-seq) library preparation from Drosophila ovaries / Nikolay V. Rozhkov
Bioinformatics analysis to identify RNA-protein interactions in oogenesis / Ravinder Singh.
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Other format(s)
Also available in an electronic version.
ISBN
9781493928507 ((hd. bd. ; : alk. paper))
1493928503 ((hd. bd. ; : alk. paper))
LCCN
2015944980
OCLC
920453641
Other standard number
10.1007/978-1-4939-2851-4
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Drosophila Oogenesis [electronic resource] : Methods and Protocols / edited by Diana P. Bratu, Gerard P. McNeil.
id
99125314441206421