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Immunosenecence : methods and protocols / edited by Albert C. Shaw, Section of Infectious Diseases, Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USA.
Format
Book
Language
English
Published/Created
New York : Humana Press, [2015]
Description
xi, 270 pages : illustrations (some color) ; 26 cm
Availability
Available Online
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1343
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Details
Subject(s)
Immunology
—
Laboratory manuals
[Browse]
Editor
Shaw, Albert C.
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1343.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 1343
Springer protocols
Summary note
In this volume, expert researchers detail protocols for evaluating the protean effects of immunosenescence on innate and adaptive immune function, including approaches to the analysis of antigen receptor repertoire. In addition, this book guides readers through cell biology and biochemical methods for analyses of telomere dysfunction, autophagy, protein oxidation, microRNAs, and DNA methylation.
Bibliographic references
Includes bibliographical references and index.
Contents
Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation
Flow Cytometry Analysis of NK Cell Phenotype and Function in Ageing
Flow Cytometric Identification of Fibrocytes in the Human Circulation
Experimental Approaches to Tissue Injury and Repair in Advanced Age
Multicolor Digital Flow Cytometry Used in Human Translational Immunology
Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates
Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry
Imaging Immunosenescence
Activation-induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine
Analyzing the Effects of Aging on CD8+ T Cell Phenotype Using Flow Cytometry
Cell-Mediated Immune Response to Influenza Using Ex Vivo Stimulation and Assays of Cytokine and Granzyme B Responses
Assays for Monitoring Macroautophagy Activity in T Cells
Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides and Protein Conformation in Biological Tissues
Monitoring the DNA Damage Response at Dysfunctional Telomeres
Single-Cell Analysis of T Cell Receptor ab Repertoire
Assessment of B Cell Repertoire in Humans
Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-throughput DNA Sequencing
Discovery of Novel MicroRNAs in Aging C. Elegans
Analysis of DNA Methylation by Pyrosequencing.
Isolation of lipid rafts from human neutrophils by density gradient centrifugation / Carl Fortin and Tamas Fülöp
Flow cytometry analysis of NK cell phenotype and function in aging / Raquel Tarazona [and others]
Flow cytometric identification of fibrocytes in the human circulation / Xinyuan Hu, Erin M. DeBiasi, and Erica L. Herzog
Experimental approaches to tissue injury and repair in advanced age / Aleah L. Brubaker, Stewart R. Carter, and Elizabeth J. Kovacs
Multicolor digital flow cytometry in human translational immunology / Samit R. Joshi, Subhasis Mohanty, and Albert C. Shaw
Flow cytometry-based methods to characterize immune senescence in nonhuman primates / Christine Meyer [and others]
Multiparameter phenotyping of human PBMCs using mass cytometry / Michael D. Leipold, Evan W. Newell, and Holden T. Maecker
Imaging immunosenescence / Feng Qian and Ruth R. Montgomery
Activation-induced cytidine deaminase and switched memory B cells as predictors of effective in vivo responses to the influenza vaccine / Daniela Frasca, Alain Diaz, and Bonnie B. Blomberg
Analyzing the effect of aging on CD8+ T-cell phenotype using flow cytometry / Min Sun Shin and Insoo Kang
Cell-mediated immune response to influenza using ex vivo stimulation and assays of cytokine and granzyme B responses / Janet E. McElhaney and Beth Gentleman
Assays for monitoring macroautophagy activity in T cells / Yair Botbol and Fernando Macian
Fluorescence-based approaches for quantitative assessment of protein carbonylation, protein disulfides, and protein conformation in biological tissues / Asish R. Chaudhuri [and others]
Monitoring the DNA damage response at dysfunctional telomeres / Rekha Rai and Sandy Chang
Single-cell analysis of T-cell receptor Αβ repertoire / Pradyot Dash, George C. Wang, and Paul G. Thomas
Assessment of B cell repertoire in humans / Yu-Chang Wu, David Kipling, and Deborah Dunn-Walters
Laboratory and data analysis methods for characterization of human B cell repertoires by high-throughput DNA sequencing / Chen Wang [and others]
Discovery of novel microRNAs in aging Caenorhabditis elegans / Alexandre de Lencastre and Frank Slack
Analysis of DNA methylation by pyrosequencing / Colin Delaney, Sanjay K. Garg, and Raymond Yung.
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Other format(s)
Also available in an electronic version.
Other title(s)
Immunosenecence, methods and protocols
ISBN
9781493929627 ((alk. paper))
1493929623 ((alk. paper))
LCCN
2015946748
OCLC
909327557
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Immunosenescence [electronic resource] : Methods and Protocols / edited by Albert C. Shaw.
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99125291285406421