Biotechnology of plant secondary metabolism : methods and protocols / edited by Arthur Germano Fett-Neto.

Format
Book
Language
English
Published/​Created
New York : Humana Press, [2016]
Description
xi, 180 pages : illustrations (some color) ; 26 cm

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Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.1405 Browse related items Request

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    Summary note
    This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • Elicitation approaches for withanolide production in hairy root culture of Withania somnifera (L.) Dunal / Ganeshan Sivanandhan [and others]
    • Stimulant paste preparation and bark streak tapping technique for pine oleoresin extraction / Thanise Nogueira Füller [and others]
    • Modified protocol for high-quality RNA extraction from oleoresin-producing adult pines / Júlio César de Lima [and others]
    • Collection of apoplastic fluids from Arabidopsis thaliana leaves / Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier
    • From plant extract to a cDNA encoding a glucosyltransferase candidate : proteomics and transcriptomics as tools to help elucidate saponin biosynthesis in Centella asiatica / Fernanda de Costa [and others]
    • Production of recombinant caffeine synthase from guarana (Paullinia cupana var. sorbilis) in Escherichia coli / Flávia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera
    • Heterologous expression and characterization of mimosinase from Leucaena leucocephala / Vishal Singh Negi and Dulal Borthakur
    • Production of aromatic plant terpenoids in recombinant baker's yeast / Anita Emmerstorfer-Augustin and Harald Pichler
    • Purification of a recombinant polyhistidine-tagged glucosyltransferase using immobilized metal-affinity chromatography (IMAC) / Fernanda de Costa [and others]
    • Western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes / Morten Egevang Jørgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier
    • Laser capture microdissection : avoiding bias in analysis by selecting just what matters / Márcia R. de Almeida and Martina V. Strömvik
    • Analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants / Inês Carqueijeiro [and others]
    • Protoplast transformation as a plant-transferable transient expression system / Patrícia Duarte [and others]
    • Design, construction, and validation of artificial MicroRNA vectors using agrobacterium-mediated transient expression system / Basdeo Bhagwat [and others]
    • Knockdown of polyphenol oxidase gene expression in potato (Solanum tuberosum L.) with artificial MicroRNAs / Ming Chi [and others].
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781493933914 ((alk. paper))
    • 1493933914 ((alk. paper))
    LCCN
    2015957398
    OCLC
    919479594
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