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Biotechnology of plant secondary metabolism : methods and protocols / edited by Arthur Germano Fett-Neto.
Format
Book
Language
English
Published/Created
New York : Humana Press, [2016]
Description
xi, 180 pages : illustrations (some color) ; 26 cm
Availability
Available Online
Online Content
Springer Nature - Complete Protocols
Copies in the Library
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Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1405
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Details
Subject(s)
Plant cells and tissues
—
Laboratory manuals
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Editor
Fett-Neto, Arthur Germano, 1967-
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Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 1405.
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Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 1405
Springer protocols
Summary note
This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.
Bibliographic references
Includes bibliographical references and index.
Contents
Elicitation approaches for withanolide production in hairy root culture of Withania somnifera (L.) Dunal / Ganeshan Sivanandhan [and others]
Stimulant paste preparation and bark streak tapping technique for pine oleoresin extraction / Thanise Nogueira Füller [and others]
Modified protocol for high-quality RNA extraction from oleoresin-producing adult pines / Júlio César de Lima [and others]
Collection of apoplastic fluids from Arabidopsis thaliana leaves / Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier
From plant extract to a cDNA encoding a glucosyltransferase candidate : proteomics and transcriptomics as tools to help elucidate saponin biosynthesis in Centella asiatica / Fernanda de Costa [and others]
Production of recombinant caffeine synthase from guarana (Paullinia cupana var. sorbilis) in Escherichia coli / Flávia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera
Heterologous expression and characterization of mimosinase from Leucaena leucocephala / Vishal Singh Negi and Dulal Borthakur
Production of aromatic plant terpenoids in recombinant baker's yeast / Anita Emmerstorfer-Augustin and Harald Pichler
Purification of a recombinant polyhistidine-tagged glucosyltransferase using immobilized metal-affinity chromatography (IMAC) / Fernanda de Costa [and others]
Western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes / Morten Egevang Jørgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier
Laser capture microdissection : avoiding bias in analysis by selecting just what matters / Márcia R. de Almeida and Martina V. Strömvik
Analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants / Inês Carqueijeiro [and others]
Protoplast transformation as a plant-transferable transient expression system / Patrícia Duarte [and others]
Design, construction, and validation of artificial MicroRNA vectors using agrobacterium-mediated transient expression system / Basdeo Bhagwat [and others]
Knockdown of polyphenol oxidase gene expression in potato (Solanum tuberosum L.) with artificial MicroRNAs / Ming Chi [and others].
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Other format(s)
Also available in an electronic version.
ISBN
9781493933914 ((alk. paper))
1493933914 ((alk. paper))
LCCN
2015957398
OCLC
919479594
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Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Biotechnology of Plant Secondary Metabolism [electronic resource] : Methods and Protocols / edited by Arthur Germano Fett-Neto.
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99125177551906421