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Mitochondrial DNA : methods and protocols / edited by Matthew McKenzie.
Uniform title
Mitochondrial DNA (Copeland)
Format
Book
Language
English
Εdition
Third edition.
Published/Created
New York : Humana Press, [2016]
©2016
Description
xi, 234 pages : illustrations (some color) ; 26 cm
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1351
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Details
Subject(s)
Mitochondrial DNA
—
Laboratory manuals
[Browse]
Mitochondrial DNA
[Browse]
Genetics
—
Technique
[Browse]
Editor
McKenzie, Matthew
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Springer protocols (Series)
[More in this series]
Methods in molecular biology (Clifton, N.J.) ; v. 1351.
[More in this series]
Springer protocols, 1949-2448
Methods in molecular biology, 1064-3745 ; v. 1351
Summary note
This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. of Mitochondrial DNA: Methods and Protocols describes protocols for detecting mutations in mitochondrial DNA, techniques to assess mitochondrial DNA damage, visualization of mitochondrial DNA in situ, detection of mitochondrial DNA nucleoids within the mitochondria, methods for analyzing mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and mitochondrial DNA copy number, the latest technologies for modifying the mitochondrial genome, and methods for the purification of proteins involved in the replication and transcription of mitochondrial DNA. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondrial DNA: Methods and Protocols, Third Edition will be useful not only for mitochondrial researchers but also for scientists studying human diseases where mitochondrial DNA variation has been recognized as an important pathogenic factor, including cancer and neurodegeneration.
Notes
Preceded by Mitochondrial DNA / edited by Jeffrey A. Stuart. 2nd ed. c2009.
Bibliographic references
Includes bibliographical references and index.
Contents
Comprehensive Mitochondrial Genome Analysis By Massively Parallel Sequencing
Analysis Of Translesion DNA Synthesis By The Mitochondrial DNA Polymerase g
Quantification Of DNA Damage By Real-Time Qpcr
Quantitation Of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single Molecule PCR
A Single-Cell Resolution Imaging Protocol Of Mitochondrial DNA Dynamics In Physiopathology, Mtrip, Which Also Evaluates Sublethal Cytotoxicity
Scalable Isolation Of Mammalian Mitochondria For Nucleic Acid And Nucleoid Analysis
Tracking Mitochondrial DNA In Situ
Analysis Of Replicating Mitochondrial DNA By In Organello Labeling And Two-Dimensional Agarose Gel Electrophoresis
Translation And Assembly Of Radiolabelled Mitochondrial DNA-Encoded Protein Subunits From Cultured Cells And Isolated Mitochondria
Analysis Of Mitochondrial DNA Copy Number And Its Regulation Through DNA Methylation Of POLGA
Engineered Mtzfns For Manipulation Of Human Mitochondrial DNA Heteroplasm
Generation Of Xenomitochondrial Embryonic Stem Cells For The Production Of Live Xenomitochondrial Mice
Import Of Fluorescent RNA Into Mitochondria Of Living Cells
Purification And Comparative Assay Of The Human Mitochondrial Replicative DNA Helicase
Expression And Purification Of Mitochondrial RNA Polymerase And Transcription Factor A From Drosophila Melanogaster
Purification And Comparative Assay Of Human Mitochondrial Single-Stranded DNA-Binding Protein
Biolayer Interferometry: A Novel Method To Elucidate Protein- Protein And Protein- DNA Interactions In The Mitochondrial DNA Replisome.
Show 14 more Contents items
Other format(s)
Also available in an electronic version.
ISBN
9781493930395 ((alk. paper))
1493930397 ((alk. paper))
LCCN
2015947227
OCLC
942840206
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Mitochondrial DNA [electronic resource] : Methods and Protocols / edited by Matthew McKenzie.
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99125291283606421